Çevresel Örneklerde Arcobacter butzleri'ye Spesifik Litik Etkili Fajların Araştırılması

Bu çalışmada, tavuk kümesleri ve sığır çiftliklerinden toplanan toprak, atık su, altlık, dışkı gibiçevresel örneklerde Arcobacter butzleri türüne spesifik fajların varlığı çift tabakalı agar yöntemikullanılarak araştırıldı. İncelenen 800 saha örneğinin iki tanesinde ilk izolasyonda gözle görülebilirnitelikte faj varlığını gösteren erimeler tespit edildi. Erimelerin olduğu agar kısmının her biri bistüriile kesilerek Tryptic Soya Broth besi yerlerine aktarıldı. İzole edilen fajları çoğaltmak amacıylayapılan daha sonraki birçok analizlerde, başlangıçta erime oluşturan iki fajın erime göstermediği veplak oluşturmadığı tespit edildi. Aynı analizler farklı kanatlı türlerinden izole edilen yedi A. butzlerisaha suşunda da denendi. Ancak herhangi pozitif bir sonuç elde edilemedi. Sonuç olarak toplanançevresel örneklerde A. butzleri’ye spesifik litik etkili fajların varlığı ile ilgili önemli ipuçları eldeedilmesine rağmen bununla ilgili kesin bir veriye ulaşılamadı. İzole edilen fajların profaj olmaihtimali dikkate alındığında, ileride yapılacak çalışmalarda bu konuya odaklanılması gerektiğikanaatine varıldı.

Investigation of Lytic Phages Speciflc to Arcobacter butzleri in Environmental Samples

In this study, the presence lytic phages specific to Arcobacter butzleri was investigated in environmental samples by using double layer agar method. In two of the 800 field samples examined, melts indicating the presence of visible phage were detected at the initial isolation. Each of the agar with melts was cut and transferred to Tryptic Soy Broth broths. In many subsequent analyzes to amplify the isolated phages, it was found that the two initially dissolving phages did not dissolve and did not form plaques. The same assays were tested in seven A. butzleri field strains isolated from different poultry species. However, no positive result was obtained. In conclusion, although there were important clues about the presence of lytic-effect phages specific to A. butzleri in the collected environmental samples, no definite data could be obtained. Considering the possibility of isolated phages to be prophage, further studies focusing on this subject are required.

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