Hasan Ceyhun MACUN, Şükrü TONBAK, Emel BIYIKLI, Ahmet Kürşat AZKUR

Buzağılarda Schmallenberg Virüs Özgül Maternal Antikor Varlığının İzlenmesi

Schmallenberg virüsü (SBV) sığır ve koyunlarda abort, ishal ve süt veriminin azalmasına neden olan bir etken olarak 2011 yılında keşfedilmiştir. Bu çalışmada, SBV ile doğal enfekte ineklerin ve kolostrumu alan buzağıların serum örneklerinde SBV özgül antikorlarının ELISA ile tespit edilebildiği sürenin belirlenmesi amaçlandı. Bir sığır çiftliğinde bulunan 148 baş inek ve buzağıdan alınan serumlarda SBV özgül antikor varlığı ELISA ile araştırıldı. İneklerde ve bu ineklere ait buzağılarda SBV özgül antikor yanıtı 8 ay boyunca aralıklarla izlendi. Toplamda 148 örnek içinde 6 tanesi yeni doğan buzağı olmak üzere 26 serum örneğinde SBV özgül antikor yanıtı pozitif olarak belirlendi (%17.56). Kolostrum almış buzağılardan 8. aya kadar serum örnekleri toplanıp SBV özgül antikor yönünden tarama yapıldı. Buzağılarda SBV seropozitifliğinin belirlenmesinin kolostrumdan kaynaklandığı görüldü. SBV seropozitif buzağıların tamamı SBV açısından 8. ayda seronegatif olarak tespit edildi ve ineklerdeki SBV seropozitiflik oranı %13.51 (20/148) olarak belirlendi. Sonuç olarak bu çalışma ile buzağılardaki SBV özgül maternal antikorların 8 aylık yaşa kadar varlığı teyit edildi. Bu neden ile SBV özgül antikor varlığı tespiti için buzağılarda yapılacak serolojik taramaların 8. aydan sonra başlaması önerildi. Aşı etkinliğinin belirlenmesi için SBV'nin koruyucu ve interfere antikor seviyesini incelendiği detaylı bir çalışma gerekli olduğu ortaya konuldu.

Monitoring Schmallenberg Virus Specific Maternal Antibodies in Calves

Schmallenberg virus (SBV), emerged in 2011, cause abortion, diarrhea and reduced milk yield in cattle and sheep. The aim of this study was to determine the duration of SBV specific maternal antibodies in calves. For this purpose, 148 sera collected from cattle and calves were tested for SBV specific antibodies with ELISA. SBV specific antibody response was monitored during 8 months in cattle and their calves. SBV seropositivity was detected in 26 out of (six of them from newborn calves) 148 samples (17.56%). Calves were tested for SBV specific antibody for 8 months after colostrum intake. Seropositivity in calves born from SBV seropositive cattle is thought to be due to colostral maternal antibodies. All SBV seropositive calves became seronegative after 8 months and SBV seropositivity of cattle was determined as 13.51% (20/148). In conclusion SBV maternal antibodies persistence for 8 months in calves is confirmed and therefore it is suggested that serological monitoring for SBV antibodies in calves should be initiated after 8 months of age. Furthermore it is necessary to investigate SBV specific protecting and interfering antibody levels for evaluation of vaccination efficiency.

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