Biochemical, Histopathological, Immunohistochemical Evaluation of Ischemic Preconditioning and Krill Oil Effects

Bu deneysel çalışmada, bir sıçan modelinde oluşturulan iskelet kası iskemi-reperfüzyon hasarında krill yağı ve iskemik önkoşullama (İÖK) etkinliği, biyokimyasal, histopatolojik ve immünohistokimyasal parametreler yardımıyla araştırıldı. Ekstremite iskemisi, femoral artere klemp konarak sağlandı. Ratlar (n=50) beş eşit gruba ayrıldı. Gruplarda ise iskemik önkoşullama protokolü ve krill yağı uygulamasının etkinliği birlikte ve ayrı olarak değerlendirildi. Malondialdehit (MDA), nitrik oksit (NO) seviyeleri ve süperoksit dismutaz (SOD), glutatyon peroksidaz (GSH-Px), katalaz (CAT) aktiviteleri ölçüldü. Işık mikroskop görüntüleri, histolojik hasar bulguları ve semikantitatif analiz bulguları ile desteklendi. KO ve İÖK uygulanan sıçanlarda İ/R grubuna göre oksidatif stresin biyokimyasal belirteçlerin iyileştiği görüldü. İ/R ve İÖK uygulanan grupların hasar seviyelerinde ise önemli oranda azalma tespit edildi. Semikantitatif HSCORE analizi ile İ/R ve İÖK gruplarına kıyasla tedavi gruplarında immünreaktivitenin arttığı ortaya konuldu. İskemik önkoşullama protokolünün ve krill yağı verilişinin iskemi-reperfüzyon hasarına karşı güçlü bir koruma sağladığı sonucuna varılmıştır.

İskemi/Reperfüzyon Modelinde İskemik Önkoşullama ve Krill Yağı Etkisinin Biyokimyasal, Histopatolojik, İmmunohistokimyasal Değerlendirmesi

The aim of the present study was to evaluate the protective effects of krill oil (KO) administration and ischemic preconditioning (IPC) protocol using biochemical, histopathological and immunohistochemical parameters in a rat model. Limb ischemia was achieved by clamping the femoral arteries. Fifty rats were divided into five groups (n=50). Effect of IPC strategy and drugreinforcement protocol was evaluated together or separately in the experimental groups. Malondialdehiyde (MDA), nitric oxide (NO), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) activities were measured. Light microscopic images were supported by histological damage score findings and semiquantitative analysis findings of ?-actin protein. Biochemical markers of oxidative stress were improved in KO and IPC groups compared with I/R group. The administration of IPC and KO resulted in a significant decrease in the damage score, 40% reduction of damage levels. Semiquantitative H-score analysis revealed that immunoreactivity increased in the treatment groups compared with I/R and IPC groups. It is concluded that ischemicpreconditioning protocol and pre-treatment with KO provides potent protection ischemiareperfusion injury.

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