Aydın SAĞLIYAN, Emre KAYA, Seval YILMAZ, Eren POLAT, Cihan GÜNAY, Mehmet Cengiz HAN, Kemal Kenan KARABULUT

Alkali Kornea Yanıklarında Amniyotik Sıvının Antioksidan Etkisinin Araştırılması

Çalışmada, amniyotik sıvının korneal alkali yanıkları üzerindeki iyileştirici etkilerini araştırmak için 40 dişi Wistar Albino sıçan kullanıldı.Sıçanlar rastgele ve eşit sayıda sıçandan oluşan 4 gruba ayrıldı. Gruplar aşağıdaki gibi tasarlanmıştır: Kontrol grubu; sıçanlara 20 gün boyunca sığır amniyotik sıvısının uygulandığı “AF” grubu; sıçanların sağ gözünde bir alkali yanığın oluştuğu ve 20 gün boyunca FTS uygulandığı “AB+SF” grubu; ve sıçanların sağ gözünde bir alkali yanığın oluştuğu ve 20 gün boyunca amniyotik sıvı uygulandığı “AB+AF” grubu.Deneyin 20. gününün sonunda ötenazi edilen hayvanların kornea dokusundaki malondialdehit (MDA) ve glutatyon (GSH) düzeyleri ile glutatyon peroksidaz (GSH-Px) aktivitesi tespit edildi. Kornea dokusundaki MDA seviyeleri ve GSH-Px aktivitesi, “AB + SF” grubunda kontrol grubuna göre anlamlı olarak arttı. Kornea dokusundaki MDA düzeyleri ‘‘AB + AF’’ ve ‘‘AB + SF’’ grupları arasında karşılaştırıldığında istatistiksel olarak anlamlı bir düşüş tespit edildi ve MDA düzeyleri kontrol grubunda elde edilenlere yaklaştı. Kornea dokusundaki GSH düzeyleri, ‘‘AB + SF’’ grubunda kontrol grubuna göre daha düşüktü ve bu iki grup arasındaki farkın anlamlı olduğu görüldü. Kornea dokusundaki GSH düzeyleri ‘‘AB + AF’’ ve ‘‘AB + SF’’ grupları arasında karşılaştırıldığında istatistiksel olarak anlamlı bir artış tespit edildi ve değerler kontrol grubuna yaklaştı. Sonuç olarak, oksidatif stres korneal alkali yanıklarda rol oynayabilir ve amniyon sıvısı onu iyileştirmede etkili olabilir.

Investigation of the Antioxidant Effects of Amniotic Fluid on Corneal Alkali Burns

In the study, 40 female Wistar Albino rats were used to investigate the healing effects of amnioticfluid on corneal alkali burns. Rats were randomly divided into 4 groups of an equal numbers of rats.The groups were assigned as follows: The control group; the group “AF”, in which the rats wereapplied with bovine amniotic fluid for 20 days; the group “AB+SF”, in which an alkali burn wasformed in the right eyes of the rats and saline was applied for 20 days; and the group “AB+AF”, inwhich an alkali burn was formed in the right eyes of the rats and amniotic fluid was applied for 20days. At the end of the 20th day of the experiment, malondialdehyde (MDA) and glutathione (GSH)levels and glutathione peroxidase (GSH-Px) activity were determined in the corneal tissue of theanimals euthanized. The MDA levels and the GSH-Px activity in the corneal tissue were found tobe increased significantly in the “AB+SF” group compared to the control group. When the MDAlevels in the corneal tissue were compared between the “AB+AF” and the “AB+SF” groups, astatistically significant decrease was determined and the MDA levels approached to those obtainedin the control group. The GSH levels in the corneal tissue were lower in the “AB+SF” groupcompared to the control group; the difference between these two groups was found to besignificant. When the GSH levels in the corneal tissue were compared between the “AB+AF” andthe “AB+SF” groups, a statistically significant increase was determined and the values approachedthe control group. In conclusion, oxidative stress may play a role in corneal alkali burns, andamniotic fluid may be effective in improving it.

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