Benzo(a)piren Uygulanan Ratlarda E Vitamini ve Selenyumun Bazı Biyokimyasal Parametreler Üzerine Etkileri

Bu çalışmada, benzo(a)piren (BaP) uygulanan ratlarda E vitamini ve selenyumun biyokimyasalparametreler üzerine olan etkileri araştırılmıştır. Bu amaçla, 80 adet dişi Wistar Albino rattan dörteşit grup oluşturulmuştur. Bu gruplar; 1. Kontrol grubu, 2. BaP grubu, 3. BaP+E vitamini grubu ve 4.BaP+selenyum grubu olarak belirlenmiştir. BaP uygulanan ratlara 10.08 mg dozunda BaP deri altıyolla bir kez verilmiştir. E vitamini uygulanan ratlara aynı dozda BaP uygulamasına ilaveten 1mg/kg dozunda E vitamini kas içi yolla gün aşırı; selenyum uygulanan ratlara ise yine aynı dozdaBaP uygulamasının yanı sıra 0.8 mg/kg dozunda selenyum intraperitoneal yolla gün aşırıuygulanmıştır. E vitamini ve selenyum uygulamaları 12 hafta süre ile devam etmiştir. Kontrolgrubuna ise Plasebo olarak intraperitonal serum fizyolojik uygulanmıştır. Çalışma sonucunda eldeedilen plazma örneklerinde kan üre nitrojeni, kreatinin, total protein, albumin, total bilirubin, direkbilirubin, kolesterol, yüksek dansiteli lipoprotein, çok düşük dansiteli lipoprotein, alanin aminotransferaz ve aspartat amino transferaz düzeyleri belirlenmiş olup, gruplar arasında üre, totalprotein, total bilirubin, yüksek dansiteli lipoprotein ve alanin amino transferaz düzeyleri bakımındanfarklılıklar tespit edilmiştir. Sonuç olarak, BaP kaynaklı olarak oluşabildiği düşünülen böbrek vekaraciğer hasarına karşı E vitamini ve selenyumun koruyucu etkileri bu çalışmada belirlenmiştir.

The Effects of Vitamin E and Selenium on Some Biochemical Parameters in Rats Exposed to Benzo(a)pyrene

In this study, the effects of vitamin E and selenium on biochemical parameters in rats treated with benzo(a)pyrene (BaP) were investigated. For this purpose, 80 female Wistar Albino rats were divided into four equal groups. These groups were; 1. Control group, 2. BaP group, 3. BaP+vitamin E group and 4. BaP+selenium group. BaP was administered subcutaneously once at a dose of 10.08 mg to rats treated with BaP. In addition to the same dose of BaP administration to rats administered vitamin E, 1 mg/kg dose of vitamin E was administered intramuscularly every other day. In addition to the same dose of BaP application, selenium at a dose of 0.8 mg/kg was administered intraperitoneally every other day. Vitamin E and selenium applications continued for 12 weeks. Intraperitoneal saline was administered to the control group as placebo. Blood urea nitrogen, creatinine, total protein, albumin, total bilirubin, direct bilirubin, cholesterol, high density lipoprotein, very low density lipoprotein, alanine amino transferase and aspartate amino transferase levels were determined in serum samples obtained as a result of the study, and urea, total protein, total bilirubin, high density lipoprotein and alanine amino transferase levels were different. In conclusion, the protective effects of vitamin E and selenium against kidney and liver damage that is thought to be caused by BaP were determined in this study.

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