Dişi kobaylarda (Cavia porcellus) duodenum mukozasının postnatal gelişimi üzerine ışık mikroskopik incelemeler

Bu çalışma, kobaylarda duodenum mukoza'sının postnatal gelişiminin ışık mikroskobik olarak ortaya konulması amacıyla yapıldı. Laparoskopik cerrahi yöntemiyle elde edilen duodenum, %10'luk formalin solüsyonunda tespit edildi. Parafin bloklardan 5-7 um kalınlığında alınan kesitlere hematoksilen-eosin ve periodic acid Schiff boyaması uygulandı. Çalışma grubu 56 kobaydan oluşmaktaydı. Duodenum'da gözlenen villi intestinales, intestinal crypt'leri döşeyen hücreler, intestinal ve duodenal bezler gibi önemli mukozal oluşumlar doğuştan mevcuttu. Yaşın ilerlemesiyle sayı ve büyüklük bakımından ilerleme kaydettikleri tespit edildi. Ondördüncü günde villus yüksekliğinde ve crypt derinliğinde önemli bir artış gözlendi. Villus'lardaki goblet hücrelerinin, crypt'lerdeki goblet hücrelerinden daha uzun ve dar oldukları belirlendi.

The light microscopical investigations on postnatal development of the duodenal mucosa of the female guinea pigs (Cavia porcellus)

This study was carried out to investigate the light microscopical changes in the duodenal mucosa of guinea pigs during postnatal development. Duodenum was obtained by laparoscopic surgery and then were fixed in buffered 10% formalin. The sections (5-7 urn) were cut in paraffin blocks and stained with haematoxylen-eosin and periodic acide Schiff stains. The study population was consisted of 56 female guinea pigs. In the duodenal mucosa, the main mucosal constituents, such as villi intestinales, lining cells of intestinal crypts, intestinal and duodenal glands were present at born. It was determined that they were increased both number and height accordance to age. It was seen that height of villus and depth of crypt were increased very important onl4 days of age. The goblet cells of villi were higher and narrower than those of crypts.

PDF

___

1. Borghi F, Petrino R, Bellora P, Gattuso G, Di Napoli A, Cellino G, Levi AC. Postnatal development of intestinal villi in the rat. Determination of villus size gradient. Panminerva Med 1994; 36: 149-151.
2. Buts JP, De Meyer R. Postnatal proximodistal development of the small bowel mucosal mass in growing rats. Biol Neonate 1981; 40: 62-69.
3. Clarke RM. The effect of growth and of fasting on the number of villi and crypts in the small intestine of the albino rat. J Anat 1972; 1: 27-33.
4. Clarke RM. The effects of age on mucosal morphology and epithelial cell production in rat small intestine. J Anat 1977; 123: 805-821.
5. Cochrane W, Davies V, Palfrey AJ and Stockwell RA. The histochemistry and electron microscopy of Brunner’s glands in the Guinea pig. J Anat 1964; 98:1-10.
6. Çalışlar T. Laboratuvar hayvanları anatomisi. Fırat Üniv Vet Fak Yay No:14. Ankara. Ankara Üniv Basımevi, 1978; 29-42.
7. Ecknauer R, Vadakel T, Wepler R. Intestinal morphology and cell production rate in aging rats. J Gerontol 1982; 37: 151-155.
8. Evans EM, Wrigglesworth KB and Pover WFR. Studies on epithelial cells isolated from Guinea pig small intestine. J Cell Biol 1971; 51: 452-464.
9. Fawcett DW. A Textbook of Histology. Eleventh edition. WB Saunders Company, 1986.
10. Friend DS. The fine structure of the Brunner’s glands in the mouse. J Cell Biol 1965; 25: 563-576.
11. Goodlad RA and Wright NA. Changes in intestinal cell proliferation, absorption capacity and structure in young, adult and old rats. J Anat 1990; 173: 109-118.
12. Holt PR, Pascal RR, Kotler DP. Effect of aging upon small intestinal structure in the Fischer rat. J Gerontol 1984; 39: 642-647.
13. Kapadia DS, Baker SJ. The effects of alterations villus shape on the intestinal mucosal surface of the albino rat. The relationship between mucosal surface area and the crypts. Digestion 1976; 14: 246-268.
14. Krause WJ and Leeson CR. The origin, development and differentiation of Brunner’s glands in the rat. J Anat 1967; 101: 309-320.
15. Mathan M, Moxey PC and Trier JS. Morphogenesis of fetal rat duodenal villi. Am J Anat 1975; 146: 73-92.
16. Merzel J and Leblond CP. Origin and renewal of the mouse small intestine. Am J Anat 1969; 124: 281-306.
17. Özeti N, Atatür M. Omurgalıların Diseksiyon Kılavuzu. İzmir. Ege Üniv. Fen Fak. Kitaplar Serisi No:70, 1975.
18. Özkan ZE, Dinç G, Aydın A. Ratlarda (Rattus norvegicus) duodenum’un morfolojik gelişimi üzerinde incelemeler. FÜ Sağlık Bil Dergisi 1998; 12:129-132.
19. Quaroni A. A crypt cell development in newborn rat small intestine. J Cell Biol 1985; 100: 1601-1610.
20. Toofanian F, Targowski SP. Morphogenesis of rabbit small intestinal mucosa. Am J Vet Res 1982; 43: 2213-2219.
21. Viguera RM, Rojas-Castaneda J, Hernandez R, Reyes G, Alvarez C. Histological characteristics of the intestinal mucosa of the rat during the first year of life. Lab Anim 1999; 33: 393-400.
22. Yu B and Chiou WS. The morphological changes of intestinal mucosa in growing rabbits. Lab Anim 1997; 31: 254-63.