Bengü TÜRKYILMAZ, Avni GÜVEN, Lale Y. AKTAŞ

Phaseolus vulgaris L.' de salisilik asit uyarımlı bazı fizyolojik ve biyokimyasal değişimler

Salisilik asit (SA), temel olarak abiyotik ve biyotik stres tepkilerinde yer alan bitki metabolizmasının endojen bir düzenleyicisidir. Farklı konsantrasyonlardaki (50, 100 ve 200 ppm) SA'in sera ve tarla koşullarında yetiştirilen Rhhseolus vulgaris L. fidelerinin bitki büyümesi ile bazı fizyolojik ve biyokimyasal Özellikleri üzerindeki etkisini araştırmak bu çalışmanın konusunu oluşturmaktadır. Fasulyede büyüme ve gelişme, yetişme koşullan ve uygulanan SA konsantrasyonuna bağlı olarak farklı nitelikler göstermektedir. Fotosentetik pigment (klorofil a, b ve karotenoidler) içerikleri açısından, serada yetiştirilen bitkilerde etkili uygulama 100 ppm SA olarak belirlenmiş ve bu uygulama her üç pigment miktarında yaklaşık %30 artışa neden olmuştur. Tarlada yetiştirilen bitkilerde ise 50 ppm SA uygulaması ile klorofil a, b ve karotenoidlerin miktarının yaklaşık %10 oranında arttığı belirlenmiştir. Sera ve tarlada yetiştirilen her iki grup bitkiye 50 ve 100 ppm S A uygulaması toplam azot içeriğini artırırken, 200 ppm S A uygulaması iki grup bitkide de kontrole göre bu değerlerde azalmaya neden olmaktadır. Sonuçlar, SA'in bu bitkinin büyüme ve azot metabolizması üzerinde uygulanan doza bağlı olarak olumlu etkisi olduğunu göstermektedir.

Salicylic acid induced some biochemical and physiological changes in Phaseolus vulgaris L.

Salicylic acid (S A) is recognized as an endogenous regulator of plant metabolism, mainly involved in biotic and abiotic stress. The objective of this study was to investigate the effects of different concentration (50, 100 and 200 ppm) of S A on greenhouse and field grown Phaseolus vulgaris L. seedlings growth, some physiological and biochemical parameters. Plant growth and development showed different results depending on growth conditions and treated SA concentration. Photosynthetic pigments (chlorophyll a, b and carotenoids) contents were all increased in greenhouse grown plants at 100 ppm SA treatment around 30%. In field grown plants, 50 ppm SA treatment caused an increase in chlorophyll a, b and carotenoids about 10%. In greenhouse and field grown plants, while 50 and 100 ppm SA treatment increased total nitrogen contents of plants, 200 ppm SA treatment caused a decrease compared to untreated control plants in both growth conditions. The results indicate that SA has a positive effects, on plant growth and nitrogen metabolism showing dose depended manner.

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1. R. F. White, Acetylsalicylic Acid (Aspirin) Induces Resistance to Tobacco Mosaic Virus in Tobacco. Virology, 99, 410-412, 1979.
2. E. R. Ward, S. J. Uknes, S. C. Williams, S. S. Dincher, D.L. Wiederhold, D. C. Alexander, P. Ahi-Goy, J. Metraux ve J.A. Ryals, Coordinate Gene Activity in Response to Agents That Induce Systemic Acquired Resistance, Plant Cell, 3, 1085-1094, 1991.
3. G. J. Kling ve M. M. Meyer, Effect of Phenolic Compounds and Indoacetic Acid on Adventitious Root Initation in Cuttings of Phaseolus aureus, Acer saccharinum, and Acer ariseum, Hort. Sci., 18, 352-354, 1983.
4. A. Jain ve H. S. Srivastava, Effect of Salicylic Acid on Nitrate Reductase Activity in Maize Seedlings, Physiol. Plant., 51, 339-342, 1981.
5. I. Raskin, Salicylate, A New Plant Hormone, Plant Physiol., 99, 799-803, 1992.
6. I. Raskin, Role of Saliciylic Acid in Plants, Ann. Rev. Plant Physiol. and Plant Mol Biology, 43, 439-463, 1992.
7. I. Raskin, Salicylic acid, In: Plant Hormones, Physiology, Biochemistry and Molecular Biology, P. J. Davies (ed.), Kluwer Acad. Pub., London, pp. 188-205, 1995.
8. R.R. Barkosky ve F.A. Einhellig, Effects of Salicylic Acid on Plant Water Relationship, J. Chem. Ecol., 19, 237-247, 1993.
9. M.P. Ramanujam, V. A. Jaleel ve G. Kumaravelu, Effect of Salicylic Acid on Nodulation, Nitrogenous Compounds and Related Enzymes of Vigna mungo, Biologia Plantarum, 41, 2, 307-311, 1998.
10. T. Senaratna, D. Touchell, E. Bunn ve K. Dixon, Acetyl Salicylic Acid Induce Multiple Stress Tolerance in Bean and Tomato Plants, Plant Growth Regul., 30, 157-161, 2000.
11. M. Cristea ve G. Drochioue, Possibilities to Stimulate Germination of Thermally Treated Wheat and Maize Seeds. Cercetari Agronomice in Moldova, 4, 49-55, 1987.
12. L. Gomez, L. Blanca ve C. S. Antonio, Evidence of the Beneficent Action of the Salicylic Acid on Wheat Genotypes Yield under Restricted Irrigation. In Proc. Scientific Meeting on Forestry, Livestock and Agriculture, Mexico., p.112, 1993.
13. L. R. Rajasekaran ve T. J. Blake, New Plant Growth Regulators Protect Photosynthesis and Enhance Growth under Drought of Jack Pine Seedlings, J. Plant Growth Regul., 18, 175-181, 1999.
14. O. H. Lowry, N. J. Rosebrough, R. J. Randall, Protein Measurement with the Folin Phenol Reagent, J. Biol. Chem., 193, 265-275, 1951.
15. U. K. Laemmli, Cleavage of Structural Proteins During the Assembly of the Head of Bacteriophage T4, Nature (London), 227, 680-685, 1970.
16. L. Steubing, Pflanzenökologisches Praktikum: Methoden und Gerate zur Bestimmung wichtiger Standörtsfaktoren, Verlag Paul Parey, Berlin, 262 p, 1965.
17. F. H. Witham, D. F. Blaydes, R. M. Devlin, Experiments in Plant Physiology, pp. 55-56, Van Nostrand Reinhold Com., New York, 1971.
18. B. Lian, X. M. Zhou, M. Miransari, D.L. Smith, Effects of Salicylic Acid on the Development and Root Nodulation of Soybean Seedlings, J. Agronomy & Crop Science, 185, 187-192, 2000.
19. X. M. Zhou, A. F. MacKenzie, C. A. Madramootoo, D. L. Smith, Effects of Stem-Injected Plant Growth Regulators, with or Without Sucrose, on Grain Production, Biomass and Photosynthetic Activity of Field-Grown Corn Plants, J. Agronomy & Crop Science, 183, 103-110, 1999.
20. B. Singh ve K. Usha, Salicylic Acid Induced Physiological and Biochemical Changes in Wheat Seedlings Under Water Stress, Plant Growth Regul., 39, 137-141, 2003.
21. G. Singh ve M. Kaur, Effect of Growth regulators on Podding and Yield of Mung Bean (Vigna radiata (L.) Wiliczek, Ind. J. Plant Physiol., 23, 366-370, 1980.
22. F. A. Einhellig, Interactive Effects of Allelochemicals and Enviromental Stress in Phytochemical Ecology: Allelochemicals. In. Mycotoxins and Insect Pheromones and Allelomones, C.H. Chou and G.R. Waller (eds.), Taiwan, pp.101-118, Academia Sinica Monograph Series