THYMOQUİNONE’NİN İNSAN MEME KANSERİ HÜCRE HATLARI ÜZERİNE ETKİLERİ

Thymoquinone (TQ), çörek otunun aktif maddesi olup, birçok kanser türlerinin tedavisinde kullanılmaktadır. Fakat insan meme kanseri hücre hatlarında henüz bir çalışma bulunmamaktadır. Dolayısıyla bu çalışmada; TQ’nın insan meme kanseri meme hatlarına etkisinin olup olmadığının araştırılması amaçlanmıştır. Çalışmada; İnsan meme adenocarcinoma hücreleri (MCF–7) Amerikan kültür koleksiyonundan (ATCC) elde edilerek kullanıldı. Thymoquinone n serisel dilüsyonları hazırlanarak mililitrede 1x105 hücre olacak şekilde 24 kuyucuklu kültür peleytlerinde 370C’de bir gece inkübe edilerek, böbrek epitelyum hücreleri ile karşılaştırıldı. Sonuçlarımızda 250 µM konsantrasyona kadar TQ’nın toksin etki göstermediği, MCF-7’nin 250 µM’da üremesini engellediği, fakat bu konsantrasyonda böbrek epitelyum hücrelerine etki etmediği gözlemlendi. Bulgularımız; TQ’nin hücre siklusunu durdurarak kanser hücrelerini öldürdüğünü göstermektedir. 250 µM konsantrasyonda kanser ücresi olmayan hücrelerde ise dayanıklılığı da görüldü.

Anahtar Kelimeler:

Thymoquinone, Çörek otu, antitümöral aktivite, Meme hücre hattı

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