Purhan Barbaros TUNCER, Mustafa Numan BUCAK, Ali BİLGEN, Serpil SAROZKAN, Hüseyin KİNET, Serhat BÜYÜKLEBLEBİCİ

The effects of different egg yolk concentrations used with soy bean lecithin-based extender on semen quality to freeze bull semen

Sarıözkan S, Tuncer PB, Bucak MN, Büyükleblebici S, Kinet H, Bilgen A. Boğa spermasının dondurulmasında soya lesitin temelli sulandırıcı ile birlikte kullanılan farklı yumurta sarısı konsantrasyonlarının sperma kalitesi üzerine etkileri. Eurasian J Vet Sci, 26, 1, 45-49 Amaç: Boğa spermasını dondurmak için soya lesitin temelli sulandırıcıya (Bioxcell®) %5 ve 10 konsantrasyonlarında santrifüj edilmiş yumurta sarısı (SYS) katıldı ve bunların çözüm sonu sperm motilite, morfolojik anormallikler ve membran bütünlüğü üzerindeki sinerjistik etkileri değerlendirildi. Gereç ve Yöntem: Her bir Simental boğadan alınan ejakulatlar (n=12) üç eşit miktara ayrıldı ve sırasıyla %5 (B5), %10 (B10) SYS eklenmiş ve hiç SYS (B0) içermeyen soya lesitin temelli sulandırıcı ile sulandırıldı. Ardından standart protokollere göre donduruldu ve çözdürüldü. Spermatozoa kryocanlılığı, in vitro çözüm sonu motilite (CASA), akrozomal ve diğer anormallikler ve plasma membran bütünlüğü (HOST) yönünden değerlendirildi. Bulgular: Simental boğalarda, dondurma ve çözdürme sonrası B5 ile sulandırılan grupta, diğer sulandırıcılarla sulandırılan gruplardan önemli derecede daha yüksek CASA motilite ve CASA progresif motilite oranı elde edilmiştir (p0.05). En yüksek VSL (p

Boğa spermasının dondurulmasında soya lesitin temelli sulandırıcı ile birlikte kullanılan farklı yumurta sarısı konsantrasyonlarının sperma kalitesi üzerine etkileri

Sariozkan S, Tuncer PB, Bucak MN, Buyukleblebici S, Kinet H, Bilgen A. The effects of different egg yolk concentrations used with soy lecithin-based extender on semen tuality to freeze bull semen. Eurasian J Vet Sci, 26, 1, 45-49 Aim: The centrifuged egg yolk (CEY) was added at concentrations of 0.5 or 10% to a defined soy lecithin-based extender (Bioxcell®) used to freeze bull semen and their synergistic effects on post-thaw sperm motility, morphological abnormalities and membrane integrity assessed. Materials and Methods: Ejaculates obtained from each Simmental bull (n:12) divided in three equal aliquots and diluted in CEY 5% (B5), 10% (B10) supplemented, and without any CEY (B0) in soy bean lecithin - based extender, respectively. Thereafter, they were frozen and thawed following a standart protocol. Sperm cryosurvival was evaluated in vitro by microscopic assessments of post-thaw sperm motility (by means of the CASA), acrosomal and other abnormalities (head, mid-pieces, and tail) and plasma membrane integrity (evaluated by HOST). Results: In Simmental bulls, semen extended with B5 had significantly higher CASA motility and CASA progressive motility than those extended with the rest of extenders after freezing and thawing (p0.05). For VSL (p

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