Purhan Barbaros TUNCER, Serpil SARİOZKAN, Mustafa Numan BUCAK, Ali BİLGEN, Huseyin KİNET, Serhat BÜYÜKLEBLEBİCİ

Evaluation of post-thaw quality of Brown-Swiss and Holstein bull semen diluted with different extenders

Sarıözkan S, Tuncer PB, Bucak MN, Büyükleblebici S, Kinet H, Bilgen A. Farklı sulandırıcılarla sulandırılmış İsviçre-Esmeri ve Holştayn boğa spermasının çözdürme sonrası kalitesinin değerlendirilmesi. Eurasian J Vet Sci, 2010, 26, 1, 51-55 Amaç: Tris + yumurta sarısı, Bioxcell® ve Optidyl® sulandırıcıları ile sulandırılan İsviçre-Esmeri ve Holştayn boğa spermasının çözdürme sonrası kalitesini değerlendirmektir. Gereç ve Yöntem: Holştayn (n=36) ve Esmer (n=36) boğalardan alınan ejakulatlar üç kısma ayrıldı ve sırasıyla Tris, Optidyl® ve Bioxcell® sulandırıcılarıyla sulandırıldı. Standart protokollere göre donduruldu ve çözdürüldü. Sulandırıcıların etkinliği, çözüm sonu sperm motilitesi, akrozomal ve toplam anormallikler ve plazma membran bütünlüğü değerlendirilerek belirlendi. Bulgular: Holştayn ırkı boğa spermasında, dondurmaçözdürme sonrası en yüksek subjektif (p

Farklı sulandırıcılarla sulandırılmış İsviçre-Esmeri ve Holştayn boğa spermasının çözdürme sonrası kalitesinin değerlendirilmesi

Sariozkan S, Tuncer PB, Bucak MN, Buyukleblebici S, Kinet H, Bilgen A. Evaluation of post-thaw quality of Brown-Swiss and Holstein bull semen diluted with different extenders. Eurasian J Vet Sci, 2010, 26, 1, 51-55 Aim: To evaluate post-thaw guality of Brown-Swiss and Holstein bull semen diluted with different extenders. Materials and Methods: Ejaculates obtained from Holstein (n=36) and Brown-Swiss (n=36) bulls were divided into three aliquots and diluted with Tris-based, Optidyl® and Bioxcell ® extenders, respectively. Thereafter they were frozen and thawed following a standard protocol. The effectiveness of freezing extenders was assessed according to postthaw sperm motility, acrosomal and total abnormalities and plasma membrane integrity. Results: With respect to Holstein bull semen, the highest percentages of sperm subjective (p

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Aboagla EM, Terada T, 2004. Effects of egg yolk during the freezing step of cryopreservation on the viability of goat spermatozoa. Theriogenology, 62, 1160-1172.
Aires VA, Hinsch KD, Mueller-Schloesser F, Bogner K, Mueller- Schloesser S, Hinsch E, 2003. In vitro and in vivo comparison of egg yolk-based and soybean lecithinbased extenders for cryopreservation of bovine semen. Theriogenology, 60, 269–279.
Amirat L, Tainturier D, Jeanneau L, Thorin C, Gerard O, Courtens JL, Anton M, 2004. Bull semen in vitro fertility after cryopreservation using egg yolk LDL: a comparison with Optidyl®, a commercial egg yolk extender. Theriogenology, 61, 895–907.
Buckett WM, Luckas MJ, Aird IA, Farquharson RG, Kingsland CR, Lewis-Jones DI, 1997. The hypo-osmotic swelling test in recurrent miscarriage. Fertil Steril, 68, 506–509.
Chatterjee S, De Lamirande E, Gagnon C, 2001. Cryopreservation alters membrane sulfhydryl status of bull spermatozoa: protection by oxidized glutathione. Mol Reprod Dev, 60, 498-506.
Forouzanfar M, Sharafi M, Hosseini SM, Ostadhosseini S, Hajian M, Hosseini L, Abedi P, Nili N, Rahmani HR, Nasr-Esfahani MH, 2010. In vitro comparison of egg yolk-based and soy lecithin based extenders for cryopreservation of ram semen. Theriogenology, 73, 480-487.
Fukui Y, Kohno H, Togari T, Hiwasa M, Okabe K, 2008. Fer- tility after artificial insemination using a soybeanbased semen extender (Andromed) in sheep. J Reprod Dev, 54, 286–289.
Gil J, Rodriguez-Irazoqui M, Lundeheim N, Soderquist L, Rodriguez-Martinez H, 2003. Fertility of ram semen frozen in Bioxcell and used for cervical artificial insemination. Theriogenology, 59, 1157-1170.
Hu JH, Li QW, Zan LS, Jiang ZL, An JH, Wang LQ, Jia YH, 2010. The cryoprotective effect of low-density lipoproteins in extenders on bull spermatozoa following freezing–thawing. Anim Reprod Sci, 117, 11-17.
Jeyendran R, Acosta V, Land S, Coulam C, 2008. Cryopreservation of human sperm in a lecithin-supplemented freezing medium. Fertil Steril, 90, 1263-1265.
Kampshmidt RF, Mayer DT, Herman HA, 1953. Lipid and lipoprotein constituents of egg yolk in the resistance and storage of bull spermatozoa. J Dairy Sci, 36, 733–742.
Moussa M, Martinet V, Trimeche A, Tainturier D, Anton M, 2002. Low density lipoproteins extracted from hen egg yolk by an easy method: cryoprotective effect on frozen-thawed bull semen. Theriogenology, 57, 1695–1706.
Revel SG, Mrode RA, 1994. An osmotic resistance test for bovine semen. Anim Reprod Sci, 36, 77-86.
Sarıözkan S, Bucak MN, Tuncer PB, Taşdemir U, Kinet H, Ulutaş PA, 2010. Effects of different extenders and centrifugation/washing on postthaw microscopicoxidative stress parameters and fertilizing ability of Angora buck sperm. Theriogenology, 73, 316–323.
Sarıözkan S, Bucak MN, Tuncer PB, Ulutas PA, 2009. Influence of Various Antioxidants on Microscopic-Oxidative Stress Indicators and Fertilizing Ability of Frozen- Thawed Bull Semen. Acta Vet Brno, 78, 463-469.
Schafer S, Holzman A, 2000. The use of transmigration and spermac stain to evaluate epididymal cat spermatozoa. Anim Reprod Sci, 59, 201-211.
Stradaioli G, Noro T, Sylla L, Monaci M, 2007. Decrease in glutathione (GSH) content in bovine sperm after cryopreservation: comparison between two extenders. Theriogenology, 67, 1249–1255.
Thun R, Hurtado M, Janett F, 2002. Comparison of Biociphos- Plus and TRIS-egg yolk extender for cryopreservation of bull semen. Theriogenology, 57, 1087-1094.
Van Wagtendonk-de Leeuw AM, Haring RM, Kaal-Lansbergen LMTE, Den Daas JHG, 2000. Fertility results using bovine semen cryopreserved with extenders based on egg yolk and soybean extract. Theriogenology, 54, 57–67.
Vishwanath R, Shannon P, 2000. Storage of bovine semen in liquid and frozen state. Anim Reprod Sci, 62, 23–53.
Wall RJ, Foote RH, 1999. Fertility of bull semen frozen and store in clarified egg yolk–Tris–glycerol extender. J Dairy Sci, 82, 817–821.
Watson PF, 1976. The protection of ram and bull spermatozoa by the low density lipoprotein fraction of egg yolk during storage at 5 oC and deep-freezing. J Termal Biol, 1, 137-141.
Watson PF, Martin IC, 1973. The response of ram spermatozoa to preperations of egg yolk in semen diluents during storage at 5 or -196oC. Aust J Biol Sci, 26, 927- 935.
White IG, 1993. Lipids and calcium uptake of sperm in relation to cold shock and preservation: A review. Reprod Fertil Dev, 5, 639-658.
Yassen AM, Foote RH, 1967. Freezability of bull semen in centrifuged or dialyzed and reconstituted buffered egg yolk extenders. J Dairy Sci, 50, 1481-1485.