Hasan ÖZEN, Musa KARAMAN, Serpil Dağ ERİNSOY, Mitat ŞAHİN

Sığır leptospirozunun patogenezinde ubiquitin ve 8-hidroksiguanozin’in rollerinin immunohistokimyasal yöntemlerle araştırılması

Karaman M, Özen H, Erginsoy SD, Şahin M. Sığır leptospirozunun patogenezinde ubiquitin ve 8-hidroksiguanozin’in rollerinin immunohistokimyasal yöntemlerle araştırılması. Eurasian J Vet Sci, 2010, 26, 1, 33-38 Amaç: Bu çalışmada, Leptospira spp. ile doğal enfekte sığırların karaciğer ve böbrek dokularında oluşan hasarda ubiquitin ve 8-hidroksiguanozin’in (8-OHG) rolleri araştırılmıştır. Gereç ve Yöntem: Leptospirozlu (n=7) ve sağlıklı kontrol grubu (n=7) hayvanlarda, bu moleküllere spesifik poliklonal antikorlar kullanılarak indirekt immunoperoksidaz metodu ile antijenlerin dokulardaki lokalizasyonları belirlendi. Bulgular: Leptospirozlu hayvanların karaciğer hepatositleri ve böbrek tubul epitellerinde ubiquitin antikoruna karşı intrasitoplazmik immun boyanma tespit edildi. 8-OHG immun boyanmasının karaciğer hepatositlerinde intrasitoplazmik ve/veya intranükleer, böbrek tubul epitellerinde ise çoğunlukla intrasitoplazmik olarak lokalize olduğu gözlendi. Öneriler: Leptospirozun patogenezinde doku hasarı oluşumunda ubiquitin ile 8-OHG’nin rol oynadığı ortaya konularak, bu moleküllere ilişkin mekanizmaları daha detaylı anlayabilmek için ayrıntılı çalışmaların gerekliliği tavsiye edilmektedir.

Immunohistochemical investigation of the role of ubiquitin and 8-hydroxyguanosine in pathogenesis of cattle leptospirosis

Karaman M, Ozen H, Erginsoy SD, Sahin M. Immunohistochemical investigation of the role of ubiquitin and 8-hydroxyguanosine in pathogenesis of cattle leptospirosis. Eurasian J Vet Sci, 2010, 26, 1, 33-38 Aim: This study was performed to investigate the role of ubiquitin and 8-hydroxyguanosine (8-OHG) expression in liver and kidney tissue damage in Leptospira-infected cattle. Material and Method: Localization of these molecules was determined by indirect immunoperoxydase method using specific polyclonal antibodies in Leptospira positive (n=7) and healthy control animals (n=7). Results: A cytoplasmic immunostaining in the hepatocytes and kidney tubule epithelia against ubiquitin antibody was detected in Leptospira-infected animals. Immunostaining against 8-OHG antibody was seen intracytoplasmic and/or intranuclear in the hepatocytes, while it was mostly intracytoplasmic in kidney tubule epithelia. Conclusion: As ubiquitin and 8-OHG were shown to play roles in pathogenesis of Leptospirosis, further detailed studies to investigate and to wholly understand the mode of mechanisms of these molecules are suggested.

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