Abigail M. BATICADOS, Waren N. BATICADOS, Lorelie A. VILLARBA, Enrique T. CARLOS, Sixto M. E.A.S. CARLOS, Paul Vincent FAJARDO

PCR assay and microscopy for examination of mixed Ehrlichia canis and Babesia spp. infection in Bomb Sniffing dogs and other canines in the National Capital Region, Philippines

Amaç: Filipinler’de köpeklerdeki Babesia ve Ehrlichia türlerinin moleküler identifikasyonu ile ilgili yayınlanmış detaylı bilgi henüz bulunmamaktadır. Bu nedenle Ulusal Başkent Bölgesi (Metropolitan Manila)’nde seçilen belediye köpeklerindeki miks Ehrlichia canis ve Babesia spp. enfeksiyonunun PZR kullanılarak belirlenmesine çalışıldı. Ayrıca farklı belediyelerde melez ve saf kan köpek gruplarının yanı sıra, yaş ve cinsiyet gruplarındaki etkenleri de tespit etmek amaçlandı. Gereç ve Yöntem: Metropolitan Manila’dan seçilen belediyelerdeki toplam 168 adet köpek kan örneği kan parazit muayenesi (BPE) ve polimeraz zincir reaksiyon(PZR) yöntemleri kullanılarak E. canis ve Babesia spp. enfeksiyonları yönünden incelendi. Bulgular: BPE ve PZR yöntemlerine tabi tutulan bütün kan örnekleri E. canis ve Babesia spp. yönünden negatif bulundu. Veriler, farklı parametreler açısından (klinik belirti), dişilerde (% 60.11), saf kan köpeklerde (%99.10) ve 0-2 yaş gruplarında (%32.94) olarak belirlendi. Öneri: Negatif sonuçlar, etkenin yokluğunu göstermemektedir. Etkenlerin vücudun diğer organlarında lokalize olması, enfeksiyonun kronik veya latent dönemde bulunması, örnek toplama zamanı, devam eden veya geçmişte uygulanan tedavi, diğer hastalıkların varlığı gibi durumlarda yanlış negatif sonuçlar ortaya çıkabilir.

Filipinler’de Ulusal Başkent Bölgesi’ndeki Bomb Sniffing cinsi ve diğer köpeklerde Ehrlichia canis ve Babesia spp. mix enfeksiyonunun PZR ve mikroskobik muayene ile incelenmesi

Aim: There are yet no published records detailing molecular identification of Babesia and Ehrlichia species in dogs in the Philippines. The detection of mixed Ehrlichia canis and Babesia spp. infection in canines of selected municipalities of the National Capital Region (Metropolitan Manila) using PCR was therefore endeavored. In addition, the research further intended to detect the individual organisms in different municipalities, age and sex categories as well as between groups of mixed and pure breed dogs. Materials and Methods: A total of 168 canine blood samples from selected municipalities of Metropolitan Manila were examined for E. canis and Babesia spp. infection using blood parasite examination (BPE) and polymerase chain reaction (PCR) assay methods. Results: All the blood samples subjected to BPE and PCR assay were found negative for E. canis and Babesia spp. The data showed that in terms of the different parameters there were more females (60.11%); purebred (88.10%) and dogs aged

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