Zafer SAYIN, Osman ERGANİŞ, Hasan Hüseyin HADİMLİ

Buzağılarda Salmonella Dublin enfeksiyonu ve otojen aşı uygulaması ile kontrolü

Hadimli HH, Sayin Z, Erganiş O.Buzağılarda Salmonella Dublin enfeksiyonu ve otojen aşı uygulaması ile kontrolü. Eurasian J Vet Sci, 2011, 27, 2, 93-98 Amaç: Bu çalışmada, bir süt ineği işletmesinde buzağılarda Salmonella enterica subsp. enterica serovar Dublin (S. Dublin) enfeksiyonunun mikrobiyolojik ve serolojik teşhisi ve izole edilen suştan hazırlanan otojen S. Dublin aşısı ile enfeksiyonun kontrolünün araştırılması amaçlandı. Gereç ve Yöntem: Ölen ve ishalli buzağıların iç organ ve dışkı örneklerinden salmonella izolasyonu için mikrobiyolojik yoklamalar yapıldı. Farklı yaş gruplarındaki hayvanlardan 447 serum örneği serolojik olarak test edildi. Saha suşundan otojen S. Dublin bakterin aşısı hazırlandı. İşletmedeki buzağılara ve süt ineklerine 6 ay aralıkla 2 yıl süreyle aşı uygulandı. Aşı uygulamalarından 1.5-2 ay sonra 4 kez 20’şer hayvandan kan ve dışkı örnekleri alındı. Serolojik yoklamalar Lam (LAT) ve Serum Aglütinasyon (SAT) testleri ile gerçekleştirildi. Bulgular: Buzağılardan izole edilen suşlar S. Dublin (1, 9, 12: g, p: -) olarak identifiye edildi. S. Dublin Lam Aglütinasyon antijeni ile 447 kan örneğinden 92’si serolojik olarak pozitif bulundu. Aşı uygulamasından sonra alınan kan örneklerinde LAT ve SAT testleri ile karşı yüksek titrede antikorlar belirlendi. İşletmede, ilk aşı uygulamasından sonra Salmonella enfeksiyonu ile ilgili herhangi bir problem ve ölüm bildirilmedi. Öneri: Buzağılarda salmonella enfeksiyonunun kontrol altına alınmasında otojen S. Dublin aşısının yardımcı olabildiği gösterildi.

The infection of Salmonella Dublin in calves and control of infection by autogenous vaccine

Aim: In this study, it was aimed to diagnose microbiologically and serologically of Salmonella enterica subsp. enterica serovar Dublin (S. Dublin) infection in calves of a dairy cow operation, and to control of infection with autogenous S. Dublin vaccine prepared from isolate of field. Materials and Methods: The microbiological examinations obtained samples of internal organs and fecal of dead or diarrhea calves were done to isolation of salmonella. Fourhundred forty seven sera samples were serologically tested in different aged groups. The autogenous bacterin S. Dublin vaccine was prepared from field strain. Vaccine was administered to calves and dairy cows at intervals 6 months for 2 years. Blood and fecal samples were collected 4 times from 20 vaccinated animals at 1.5-2 month after vaccination. Serological examinations were done by Plate (LAT) and Serum Agglutination (SAT) tests. Results: The strains of Salmonella ssp. isolated from calves were identified as S. Dublin (1, 9, 12: g, p: -). 92 of 447 sera samples were found serologically positive. Higher antibodies titter in blood samples were detected by LAT and SAT. After the first vaccination, any problem and death related to Salmonella infection has not been reported in farm. Conclusion: The study showed that the autogenous S. Dublin vaccine could be help for control of S. Dublin infection in calves.

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