Zafer SAYIN, Osman ERGANIS, Kursat KAV, Hasan Huseyin HADİMLİ

The production and development of vaccines for Ornithobacterium rhinotracheale infection in commercial broilers

Amaç: Çalışmanın amacı bivalan inaktif Ornithobacterium rhinotracheale (O. rhinotracheale) aşıları hazırlamak, kan serumlarında antijenlere karşı antikorların titrelerini ölçmek ve etçi piliçlerde O. rhinotracheale aşılarının etkinliklerini belirlemektir. Gereç ve Yöntem: Bivalan inaktif O. rhinotracheale aşıları; alüminyum hidroksit (Al[OH]3), mineral yağlı (MO), alüminyum hidroksit + ginseng (Al[OH]3 + G) ve mineral yağ + ginseng (MO + G) adjuvantları kullanılarak O. rhinotracheale serotip A ve B’den hazırlandı. Sterilite ve zararsızlık testlerinden sonra, etçi piliçlerde (ilk gün 0.1 mL doz ile bir kez aşılama ile) aşıların laboratuvar etkinlikleri (çelınç/koruma ve serolojik potens) kontrol edildi. Bulgular: Çelınç sonuçlarına gore; etçi piliçlerde bütün aşıların %100 etkili olduğu bulundu. Adjuvant olarak mineral yağ ve ginseng (MO + G) içeren aşı diğerlerine göre belirgin olarak daha yüksek humoral immune cevap oluşturdu. Öneri: Bu çalışmada, birinci günde etçi piliçlerin aşılanması O. rhinotracheale enfeksiyonlarına karşı etkili şekilde koruyabildiği gösterilmiştir.

Piliçlerde Ornithobacterium rhinotrachealae’ye karşı aşı üretimi ve geliştirilmesi

Aim: The purpose of this study was to prepare bivalent inactive Ornithobacterium rhinotracheale (O. rhinotarcheale) bacterin vaccines, to measure the levels of antibodies against antigens in blood sera and to determine the efficacies of different O. rhinotracheale vaccines in broilers. Materials and Methods: The bivalent inactivated O. rhinotracheale bacterin vaccines were prepared from O. rhinotracheale serotype A and B strains using aluminium hydroxide (Al[OH]3), mineral oil (MO), aluminium hydroxide + ginseng (Al[OH]3 + G) and mineral oil + ginseng (MO + G). After the sterility and the safety tests, laboratory efficiencies of vaccines (challange/protection and serological potency) were controlled on the broilers (vaccinated at first day with a dose of 0.1 mL). Results: According to the challenge results, all the vaccines were found to be effective as 100% for broilers. The vaccine containing mineral oil and ginseng (MO + G) as adjuvant induced significantly greater humoral immune response to the antigens than others. Conclusion: The study showed that vaccination of broilers chicken at the first day age may effectively protect against O. rhinotracheale infections.

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