Özgür KAYNAR, Armağan Erdem ÜTÜK, İbrahim BALKAYA, Fatma Çiğdem PİŞKİN

Theileria equi seropozitif ve seronegatif atların serum lipid ve protein profilleri

Amaç: Bu çalışma Theileria equi seropozitif ve seronegatif atlar üzerinde, serum protein ve lipid profillerini ortaya koymak amacıyla yürütülmüştür. Gereç ve Yöntem: Theileria equi seropozitif ve seronegatif atlar IFAT ile test edilerek belirlendi. Bu atlara ait kan serumlarında SDS-PAGE ve HPTLC analizleri yapılarak sırasıyla serum protein ve lipit profilleri ortaya çıkarıldı ve bu analiz sonuçlarına göre seropozitif ve seronegatif atlar arasındaki farklılıklar ortaya konulmaya çalışıldı. Bulgular: Seropozitif hayvanların 23, 52, 103, 176, 200 ve 240 kDa ağırlığındaki proteinlerinde önemli oranda artış olduğu tespit edildi. Ayrıca hem sağlıklı hem de T. equi seropozitif hayvanların albümin miktarları benzer düzeylerde olmasına karşın T. equi seropozitif hayvanların globülin miktarlarının arttığı görüldü. Serum lipid profilinde ise seropozitif hayvanların genel bir lipoliz durumunda oldukları, toplam lipid miktarı içinde özellikle hidrokarbon oranının azaldığı ve diaçilgliserol düzeyinin arttığı belirlendi. Yine seropozitif hayvanlarda fosfolipid miktarında hafif artış ve kolesterol oranında ise hafif bir düşme tespit edildi. Öneri: Protein ve lipid profillerindeki bu değişiklikler, hayvanların akut enfeksiyonu atlatmalarına rağmen hala tam olarak iyileşemediklerini göstermektedir.

Serum lipid and protein profiles of Theileria equi seropositive and seronegative horses

Aim: This study aims at determining serum protein and lipid profiles in Theileria equi seropositive and seronegative horses. Materials and Methods: Theileria equi seropositivity and seronegativity at horses were tested by IFAT. Having analyzed the blood sera of horses by SDS-PAGE and HPTLC, serum protein and lipid profiles were detected respectively. Differences between seropositive and seronegative horses were tried to be determined based on the results obtained by said analysis. Results: It was detected that there was a significant increase in 23, 52, 103, 176, 200 and 240 kDa protein levels of seropositive horses. Additionally, globulin levels of T. equi seropositive animals were observed to have increased, although albumin levels of both healthy and T. equi seropositive horses were similar. At serum lipid profile, seropositive horses were observed to be at overall lipolysis; among the total amount of lipid, particularly reduced hydrocarbon rates as well as increased diacylglycerol levels were detected. A slight increase in phospholipids and a slight decrease in cholesterol levels were found in seropositive horses. Conclusion: Mentioned changes at both protein and lipid profiles suggest that these horses have not fully recovered despite the fact that they survived acute infection.

PDF

___

Ali S, Sugimoto C, Matsuda M, Sugiura T, Kanemaru T, Onuma M, Kamada M, 1993. Protein characterization of Babesia equi piroplasms isolated from infected horse erythrocytes. Parasitol Res, 79, 639-643.
Anonim, 2009. Equine Piroplasmosis Backgrounder (August 25, 2006), American Veterinary Medical Association, http://www.avma.org/reference/backgrounders/ equine_piroplasmosis_bgnd.pdf, Erişim tarihi; Aralık 2009.
Avarzed A, Igarashi I, De Waal DT, Kawai S, Oomori Y, Inoue N, Maki Y, Omata Y, Saito A, Nagasawa H, Toyoda Y, Suzuki N, 1998. Monoclonal Antibody against Babesia equi: Characterization and Potential Application of Antigen for Serodiagnosis. J Clin Microbiol, 36, 1835-1839.
Brüning A, 1996. Equine piroplasmosis an update on diagnosis treatment and prevention. Br Vet J, 152, 139-151.
Elke HD, 2007. Applied Thin-Layer Chromatography, 2nd ed., Wiley-VCH Verlag GmbH & Co., KGaA, Weinheim, Germany.
Frerichs WM, Holbrook AA, Johnson AJ, 1969. Equine piroplasmosis: Production of antigens for the complement fixation test. Am J Vet Res, 30, 1337-1341.
Kumar S, Malhotra DV, Nichani AK, 2002. Identification of immunoreactive polypeptides of Babesia equi parasite during immunization. Vet Parasitol, 107, 295-301.
Levine ND, 1985. Vet. Protozool., 1th edition, Iowa State University Press, Ames, USA.
Mehlhorn H, Schein E, 1998. Redescription of Babesia equi Laveran, 1901 as Theileria equi Mehlhorn, Schein 1998. Parasitol Res, 84, 467-475.
Mimioğlu M, Göksu K, Sayın F, 1969. Veteriner ve Tıbbi Protozooloji II, AÜ Vet Fak Yay 248, AÜ Basımevi, Ankara, Türkiye.
OIE, 2008. Equine piroplasmosis. http//:www.oie.int/eng/ normes/manual/2008/pdf/02.05.08_EQUINE_PIROPLASMOSI.
Reiner W, 2005. Electrophoresis in Practice, 4th edition, WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany.
Ristic M, Oppermann J, Sibinovic S, Phillips TN, 1964. Equine piroplasmosis - A mixed strain of Piroplasma caballi and Piroplasma equi isolated in Florida and studied by the fluorescent- antibody technique. Am J Vet Res, 25, 15- 23.
Soulsby EJL, 1986. Helmints, Arthropods and Protozoa of Domesticated Animals, Baillare Tindall, London, UK.
Tenter AM, Friedhoff KT, 1986. Serodiagnosis of experimental and natural Babesia equi and B.caballi infections. Vet Parasitol, 20, 49-61.
Weiland G, 1986. Species-Specific serodiagnosis of equine piroplasma infections by means of Complement Fixation Test (CFT), Immunofluorescence (IIF) and Enzyme- Linked Immunosorbent Assay (ELISA). Vet Parasitol, 20, 43-48.