Mehmet Bozkurt ATAMAN, Ali Doğan ÖMÜR, Pınar Peker AKALIN, Caner ÖZTÜRK, Şükrü GÜNGÖR, Kenan ÇOYAN, Mustafa Numan BUCAK, Nuri BAŞPINAR

Koç spermasının ekilibrasyon ve dondurma-çözdürme sonrası spermatolojik ve biyokimyasal parametreleri üzerine lipoik asitin etkisi

Amaç: Koç sperması sulandırıcısına katılan farklı dozlardaki lipoik asitin spermanın ekilibrasyon ve dondurma sonrası bazı spermatolojik ve biyokimyasal parametreler üzerine etkilerinin araştırılması amaçlandı. Gereç ve Yöntem: Araştırmada 6 baş ergin Konya Merinosu koçlara ait ejakülatlar kullanıldı. Ejakülatlar 4 eşit hacme bölünerek lipoik asit 1, 2, 4 mM içeren ve içermeyen (kontrol) Tris sulandırıcısıyla mL’de yaklaşık 400 x 106 spermatozoa olacak şekilde 37 0C’ta sulandırıldı ve donduruldu. Bulgular: Ekilibrasyon sonrası lipoik asitin kontrol gruplarına göre sperm parametrelerinde etkisi görülmezken, dondurma-çözdürme sonrası numunelerde lipoik asit 1 mM (%74.3), kontrol (%62.5) ve lipoik asit 4 mM (%65.0) gruplarına göre motilite yönünden önemli derecede iyileştirme sağladı (p0.05). Öneri: Koç spermasına 1mM dozda lipoik asit ilavesi dondurma-çözdürme sonrası motiliteyi arttırmaktadır. Bununla birlikte lipoik asitin farklı sulandırıcılarda ve hayvan türleri-ırklarında doz denemelerinin yapılması gerekmektedir.

Effect of lipoic acid on spermatological and biochemical parameters following equilibration and freeze-thawing of ram semen

Aim: The aim of this study was to investigate the effects of different doses of lipoic acid added into dilution extender, following equilibration and freeze-thawing of ram semen on spermatologic and biochemical parameters. Materials and Methods: The ejaculates collected from 6 mature Konya Merino rams were used in the research. Ejaculates containing approximately 400x106 spermatozoa were diluted at 37 0C with the Tris-based extender containing lipoic acid at the doses of 1, 2, 4 mM and no additive (control), and then, they were frozen. Results: After equilibration, all doses of lipoic acid did not affect the sperm parameters whereas lipoic acid 1 mM (74.3%) ameliorated motility compared to both control (62.5%) and lipoic acid 4 mM (65.0%) (p0.05). Conclusion: Addition of 1 mM lipoic acid to ram semen increases the motility after freeze-thawing process. However, dose treatments of lipoic acid in different extenders and animal species-breeds are required to be performed.

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