Uçkun Sait UÇAN, Aslı SAKMANOĞLU, Ali USLU, Zafer SAYIN, Ecem Oglakci CANSOY

Nigella sativa soğuk bası yağının aşı adjuvantı olarak değerlendirilmesi

Amaç: Organik ve bitkisel kökenli, düşük düzeyde toksik olan, daha ucuz ve etkili bir adjuvant geliştirilmesi amacıyla soğuk bası Nigella sativa yağı(SBNSY) ve bu yağın temel bileşenlerinden timokiononun adjuvant potansiyeli incelendi. Gereç ve Yöntem: SBNSY veya timokionon ile ovalbumin verilerek oluşturulan spesifik antikorların ölçümü değerlendirildi. Swiss al bino fareler (n=36) A0 (kontrol), A1 (timokionon), A2 (SBNSY), A3 (içeriği; timokionon miktarında % 10 oranında arttırım sureti ile değiştirilmiş olan SBNSY) ve A4 (ticari adjuvant, Al(OH)3) olmak üzere farklı şekillerde verildi. Etkinin değerlendirilmesi, immünizas yondan sonra ovalbumine karşı oluşan spesifik antikoların I-ELISA ile ölçümü yolu ile ve laboratuvarımızda yapıldı. Bulgular: Serum ve konjugatın optimal sulandırmaları sırası ile 1/200 ve 1/40.000 olarak bulundu. 450 nm’de okunan ortalama OD değerleri kontrol (A0), A1, A2, A3 ve A4 grupları için sırası ile 0,043 (± 0,002), 0,668 (± 0,074), 0,644 (± 0,018), 0,675 (± 0,066), ve 0,745 (± 0,09) idi. Öneri: Her üç formulasyon da (A1, A2, A3), kontrol grubuna göre ovalbumine karşı sıvısal immün yanıtın oluşturulmasında ticari ad juvant (A4) formulasyonu kadar etkili bulundu (p > 0,05). Farede, bu çalışmada denenen her bir Nigella sativa temelli adjuvant adayının potansiyel olarak Al(OH)3’e alternatif olabileceği kanaatine varıldı.

Evaluation of Nigella sativa's cold press oil as vaccine adjuvant

Aim: In order to identify an organic and plant based, less toxic, cheaper and more effective adjuvant, a cold pressed oil of Nigella sativa(CPNSO) and one of the essential components of Nigella sativa oil(NSO), thymoquinone (Thymoquinone ) were investigated as adjuvant candidates.Materials and Methods: Adjuvant potentials of the both were measured by examining specific antiibody titers to ovalbumin given inpresence of either of CPNSO or thymoquinone, in vivo. Such potentials of thymoquinone alone (A1), CPNSO (A2), mCPNSO (experimentally formed by addition of thymoquinone to CPNSO making upmCPNSO that was contained 10% more thymoquinone than originalCPNSO did; A3) and Al(OH)3 (A4) in presence of ovalbumin in Swissalbino mice (n=36, female). The effects were determined by measuring anti-ovalbumin antibodies after immunization to ovalbumin byhome-made ELISA in mice.Results: Sera and conjugate were optimally diluted 1/200 and1/40.000, respectively. Mean OD values at 450 nm of the groupscontrol (A0), A1, A2, A3 and A4 were 0,043 (± 0,002), 0,668 (±0,074), 0,644 (± 0,018), 0,675 (± 0,066), and 0,745 (± 0,09), respectively.Conclusion: By comparison control, all three (A1, A2, A3) of the testformulations were found to be as effective as commercial (A4) formulation in triggering humoral response to ovalbumin (p > 0,05).Therefore, each of Nigella sativa based adjuvant candidates has analternative potential to Al(OH)3 in the mouse

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