Sarah AKAR, Nilay ÇÖPLÜ, Mehmet Cengiz BALOĞLU, Yasemin Celik ALTUNOĞLU, Orhan CORUM, Anfal Izaldeen ALKATEEB, Muammer KİRAZ, Enis Fuat TÜFEKCI

Hatay bölgesinden çakşır otunun (Ferula elaeochytris Korovin) antimikrobiyal ve anti-quorum sensing aktiviteleri

Amaç: Günümüzde giderek artan antibiyotik direnci, bilim dünyasını yeni antimikrobiyal moleküllerin keşfine veya alternatif mücadele yöntemlerinin geliştirilmesi üzerine odaklamıştır. Bu alternatif mücadele yöntemlerinden bir tanesinin bakterilerde quorum sensing (QS; çoğunluğu algılama) inhibisyonu olacağı öngörülmektedir. Çünkü QS sistemi bakterilerde virülans faktörlerinin sentezinde önemli bir rol oynar. Medikal öneme sahip birçok bitkinin umut verici antimikrobiyal ve anti-QS aktivitelere sahip olduğu bilinmektedir. Bu bitkilerden birisinin de Anadolu'da yıllardır yaygın olarak kullanılan çakşır otu (Ferula elaeochytris Korovin) olabileceğini düşünmekteyiz. Bu nedenle, bu çalışmada F. elaeochytris Korovin’in kök özütünün antimikrobiyal ve anti-QS aktivitelerinin araştırılması amaçlanmıştır. Gereç ve Yöntem: Özütün antimikrobiyal aktivitesi, çeşitli mikroorganizma lara karşı disk difüzyon yöntemi ile taranmıştır. Özütün etkili suş veya suşla ra karşı minimum inhibitör konsantrasyonu (MİK) ve minimum bakterisidal konsantrasyonu (MBK) değerleri sıvı mikrodilüsyon yöntemi ile belirlenmiştir. Anti-QS aktivite, Pseudomonas aeruginosa ve Chromobacterium violaceum bi yoraportör suşlarında sırasıyla piyosiyanin ve viyolasin pigmentleri üretimi nin inhibisyonu üzerine araştırılmıştır. Bulgular: Özütün sadece Staphylococcus aureus suşuna karşı antimikrobiyal aktivitesi (zon çapı = 9.3±0.6 mm) tespit edilmiştir. MİK ve MBK değerleri sı rasıyla 4.4±1.9 mg/mL ve >105 mg/mL olarak saptanmıştır. Viyolasin üretimi inhibisyonu belirlenmemiştir. Özütün MİK altı konsantrasyon değerleri (1.64 µg/mL ve 3.28 µg/mL) bakteri üremesini baskılamadan piyosiyanin üretimini sırasıyla %60 ve %82 oranında inhibe etmiştir. Öneri: Bu çalışmanın sonuçları F. elaeochytris Korovin'in kök özütünün an tistafilokokal ve anti-QS ajanların geliştirilmesi için iyi bir aday olabileceğini göstermektedir

Antimicrobial and anti-quorum sensing activities of giant fennel (Ferula elaeochytris Korovin) from the Hatay region

Aim: Today, increasing antibiotic resistance has focused the science world onthe discovery of new antimicrobial molecules or the development of alternative methods of struggle. One of the alternative methods is thought to be inhibition of bacterial quorum sensing (QS). Because the QS system performs acrucial part in the synthesis of virulence factors in bacteria. Numerous medicinal plants are known to have promising antimicrobial and anti-QS activities.One of these plants may be giant fennel (Ferula elaeochytris Korovin), whichhas been extensively used in Anatolia for years. Therefore, it was aimed to investigate the antimicrobial and anti-QS activities of the root extract of F. elaeochytris Korovin in this study. .Materials and Methods: The antimicrobial activity of the extract was screened by disc diffusion assay against various microorganisms. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) ofthe extract against sensitive strain(s) were determined using broth microdilution assay. Anti-QS activity investigated on inhibition of violacein and pyocyanin production in Chromobacterium violaceum and Pseudomonas aeruginosabioreporter strains, respectively.Results: The extract exhibited the antimicrobial activity against only Staphylococcus aureus (zone of inhibition (ZOI) = 9.3±0.6 mm). The MIC and theMBC values were determined as 4.4±1.9 mg/mL and >105 mg/mL, respectively. No inhibition of violacein production was detected. Pyocyanin productionwas reduced by 60% and 82% at sub-MIC concentrations (at 1.64 mg/mL and3.28 mg/mL, respectively) as against the control (p

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Akaberi M, Iranshahy M, Iranshahi M, 2015. Review of the traditional uses, phytochemistry, pharmacology and toxicology of giant fennel (Ferula communis L. subsp. communis). Iran J Basic Med Sci, 18(11), 1050-1062.
Altundag E, Ozturk M, 2011. Ethnomedicinal studies on the plant resources of east Anatolia, Turkey. Procedia – Social and Behavioral Sciences, 19, 756-777.
Asili J, Sahebkar A, Bazzaz FBS, Sharifi S, et al., 2009. Identification of essential oil components of Ferula badrakema fruits by GC-MS and 13C-NMR methods and evaluation of its antimicrobial activity. J Essent Oil-Bear Plants, 12(1), 7-15.
Eberl L, Winson MK, Sternberg C, Stewart GS, et al., 1996. Involvement of N-acyl-L-hormoserine lactone autoinducers in controlling the multicellular behaviour of Serratia liquefaciens. Mol Microbiol, 20(1), 127–136.
Essar DW, Eberly L, Hadero A, Crawford IP, 1990. Identification and characterization of genes for a second anthranilate synthase in Pseudomonas aeruginosa: interchangeability of the two anthranilate synthases and evolutionary implications. J Bacteriol, 172(2), 884-900.
Ghasemi Y, Faridi P, Iraj M, Mohagheghzadeh A, 2005. Ferula gummosa fruits: An aromatic antimicrobial agent. Chem Nat Compd, 41(3), 311-314.
Güzel Y, Güzelşemme M, Miski M, 2015. Ethnobotany of medicinal plants used in Antakya: A multicultural district in Hatay Province of Turkey. J Ethnopharmacol, 174, 118-152.
Hentzer M, Givskov M, 2003. Pharmacological inhibition of quorum sensing for the treatment of chronic bacterial infections. J Clin Invest, 112(9), 1300-1307.
Iranshahi M, Hassanzadeh M, Bazzaz F, Sedigheh B, et al., 2008. High content of polysulphides in the volatile oil of Ferula latisecta Rech. F. et Aell. fruits and antimicrobial activity of the oil. J Essent Oil-Bear, 20(2), 183-185.
Jiang Q, Chen J, Yang C, Yin Y, et al., 2019. Quorum sensing: A prospective therapeutic target for bacterial diseases. BioMed Res Intern, 2019, 2015978.
Jomehpour N, Eslami G, Khalili M, 2016. The effect of Ferula assa-foetida L and Carum copticum hydroalcoholic extract on the expression levels of Staphylococcus aureus genes involved in quorum sensing. Jundishapur J Microbiol, 9(10), e33879.
Kalia M, Yadav VK, Singh PK, Sharma D, et al., 2015. Effect of cinnamon oil on quorum sensing-controlled virulence factors and biofilm formation in Pseudomonas aeruginosa. PLoS One, 10(8), e0135495.
Khambhala P, Verma S, Seshadri S, Kothari V, 2016. Inhibition of bacterial quorum-sensing by Ferula asafoetida essential oil. Adv Genet Eng, 5, 2.
Khoury M, Beyrouthy ME, Eparvier V, Ouaini N, et al., 2017. Chemical diversity and antimicrobial activity of the essential oils of four Apiaceae species growing wild in Lebanon. J Essent Oil-Bear, 30(1), 25-31.
Kose E, Aktas O, Deniz I, Sarikurkcu C, 2010. Chemical composition, antimicrobial and antioxidant activity of essential oil of endemic Ferula lycia Boiss. J Med Plant Res, 4(17), 1698-1703.
Lade H, Paul D, Kweon JH, 2014. Quorum quenching mediated approaches for control of membrane biofouling. Int J Biol Sci, 10(5), 550-565.
Lin J, Opoku AR, Geheeb-Keller M, Hutchings AD, et al., 1999. Preliminary screening of some traditional zulu medicinal plants for anti-inflammatory and anti-microbial activities.
J Ethnopharmacol, 68(1-3), 267-274. Marketon MM, Glenn SA, Eberhard A, González JE, 2003. Quorum sensing controls exopolysaccharide production in Sinorhizobium meliloti. J Bacteriol, 185(1), 325-331.
McClean K, Winson M, Fish L, Taylor A, et al., 1997. Quorum sensing and Chromobacterium violaceum: exploitation of violacein production and inhibition for the detection of N-acylhomoserine lactones. Microbiology, 143(Pt.12), 3703-3711.
Morohoshi T, Kato M, Fukamachi K, Kato N, et al., 2008. N-acylhomoserine lactone regulates violacein production in Chromobacterium violaceum type strain ATCC 12472. FEMS Microbiol Lett, 279(1), 124-130.
Nellums LB, Thompson H, Holmes A, Castro-Sánchez E, et al., 2018. Antimicrobial resistance among migrants in Europe: a systematic review and meta-analysis. Lancet Infect Dis, 18(7), 796-811.
Ohtani K, Hayashi H, Shimizu T, 2002. The luxS gene is involved in cell-cell signalling for toxin production in Clostridium perfringens. Mol Microbiol, 44(1), 171–179.
Ozek G, Ozek T, Iscan G, Baser KHC, et al., 2008. Composition and Antimicrobial Activity of the Oils of Ferula szowitsiana DC. from Turkey. J Essent Oil-Bear, 20(2), 186-190.
Rice SA, Koh KS, Queck SY, Labbate M, et al., 2005. Biofilm formation and sloughing in Serratia marcescens are controlled by quorum sensing and nutrient cues. J Bacteriol, 187(10), 3477–3485.
Sargin SA, 2015. Ethnobotanical survey of medicinal plants in Bozyazı district of Mersin, Turkey. J Ethnopharmacol, 173, 105-126.
Sepahi E, Tarighi S, Ahmadi FS., Bagheri A, 2015. Inhibition of quorum sensing in Pseudomonas aeruginosa by two herbal essential oils from Apiaceae family. J Microbiol, 53(2), 176–180.
Shapiro SS, Wilk MB, 1965. An analysis of variance test for normality (complete samples). Biometrika, 52, 591-611
The European Committee on Antimicrobial Susceptibility Testing, 2020. Disk diffusion method, version 8.0. http:// www.eucast.org/ast_of_bacteria/disk_diffusion_methodology/
Towse A, Hoyle CK, Goodall J, Hirsch M, et al., 2017. Time for a change in how new antibiotics are reimbursed: Development of an insurance framework for funding new antibiotics based on a policy of risk mitigation. Health Policy, 121(10), 1025-1030.
Wiegand I, Hilpert K, Hancock RE, 2008. Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nat Protoc, 3(2), 163-175.