Enver YAZAR, Rahmi CANBAR, Merve İDER, Devran COŞKUN, Mustafa Sedat ARSLAN

Koyunlara interferon alfa uygulamasının sitokinler üzerine etkisi

Amaç: Bu çalışmanın temel amacı, sağlıklı koyunlara parenteral rekombinant insan interferon (rHuIFN)-α2a uygulamasının serum tümör nekroz faktör (TNF)-α, interlöykin (IL)-6 ve IL-10 seviyeleri üzerindeki etkisini belirlemektir. Ayrıca sağlıklı koyunlarda temel fizyolojik parametreler (vücut ısısı, nabız, solunum hızı), hemogram ve kan gazı parametrelerine etkisini tespit etmektir. Gereç ve Yöntem: Bu çalışmada 10 Merinos koyuna 9.000.000 IU rHuIFN-α2a deri altı yolla uygulandı. Kan örnekleri uygulamadan önce (0 saat) ve sonra 4, 8, 12, 24, 48, 72, 96 ve 120 saatlerde alındı. Aynı örnekleme zamanlarında vücut ısısı, nabız ve solunum hızı da belirlendi. Serum örneklerinden koyun spesifik TNF-α, IL-6 ve IL-10 seviyeleri ELISA okuyucu ile ölçüldü. Hemogram ve kan gazı parametreleri sırasıyla tam kan hücresi sayım cihazı ve kan gazı analiz cihazında ölçüldü. Bulgular: rHuIFN-α2a uygulaması sonrasında TNF-α ve IL-10 konsantrasyonları 96. saatte pik düzeye (p0.05). Ayrıca vücut ısısı ve pO2 düzeylerinde geçici artışlar (p

The effect of interferon alpha administration on cytokine levels in sheep

Aim: The primary aim of this study was to determine the effect ofparenteral recombinant human interferon (rHuIFN)-α2a administration on serum tumor necrosis factor (TNF)-α, interleukin (IL)-6and IL-10 levels in healthy sheep. In addition, the main physiologicalparameters (rectal temperature, pulse rate, respiratory rate), hemogram and blood gas parameters were determined, as well.Materials and Methods: In this study, 9.000.000 IU rHuIFN-α2awas administered subcutaneously in 10 Merinos sheep. Blood samples were taken before (0 hours) and after at 4, 8, 12, 24, 48, 72, 96and 120 hours. Rectal temperature, pulse and respiratory rate werealso determined at the same sampling times. Sheep-specific TNF-α,IL-6 and IL-10 levels were determined from serum samples by theELISA reader. Hemogram and blood gas parameters were measuredby complete blood cell counter and blood gas analyzer, respectively.Results: After rHuIFN-α2a treatment, TNF-α and IL-10 concentrations reached peak levels (p0.05). On the other hand, a temporary increase in rectal temperature and pO2 levels(p

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