Streptococcus equi zooepidemicus izolatlarının biofilm üretiminin değerlendirilmesi

Amaç: Streptococcus equi zooepidemicus izolatlarının biofilm üretme kapasitelerinin ölçülmesi amaçlandı. Gereç ve Yöntem: Selçuk Üniversitesi Veteriner Fakültesi Mikrobiyoloji Anabilim Dalı kültür koleksiyonundan sağlanan kırk adet Streptococcus equi zooepidemicusizolatı Kongo kırmızısı agar, Kristal moru boyama ve taramalı electron mikroskobu (TEM) olmak üzere 3 farklı yöntem ile biofilm oluşturma yönünden değerlendirildi. Bulgular: Kongo kırmızısı agar ile Kristal moru boyama sonuçları arasında uyumsuzluk vardı. Otuz bir pozitif suşun 19’u Kongo kırmızısı agarda kuvvetli pozitif verirken 30’u Kristal moru boyama ile kuvvetli pozitif bulundu. Biofilm üretimi TEM ile doğrulandı. Öneri: Ülkede at hekimliği hizmeti veren klinisyenlerin, Streptococcus equi zooepidemicus etkenlerinin in vitro şartlarda biofilm oluşturabileceğinin farkında olmaları önerilir.

The evaluation of biofilm production by Streptococcus equi zooepidemicus isolates

Aim: The purpose of this study is to measure biofim production capabilities of Streptococcus equi zooepidemicus isolates.Materials and Methods: Forty one horse strains of Streptococcus equi zooepidemicus from the laboratory stock collection of Microbiology Department of the Veterinary Faculty, Selçuk University wereevaluated by the biofilm formation using three different methods:Congo red agar, crystal violet staining method and scanning electronmicroscopy (SEM).Results: Incompatibility between the results obtained from Congored agar and crystal violet staining methods was detected. Nineteen out of 31 positive strains gave strong positive on Congo red agarwhile 30 of them were considered as strongly biofilm producingstrains in crystal violet staining method. The biofilm production wasconfirmed and the biofilm layers were detected by using the SEMmicroscope.Conclusion: Equine clinicians in this Country should be aware ofthat Streptococcus equi zooepidemicus isolates are capable of forming biofilm in vitro.

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