Mehmet Salih KAYA, Nurettin AYDİLEK, Faruk BOZKAYA, Mehmet Osman ATLİ, Aydın GUZELOGLU, Seyit Ali KAYİS

Effect of long term heat stress and dietary restriction on the expression of small heat shock protein (sHSP) genes in rat liver tissue

Amaç: Bu çalışmanın amacı uzun süreli sıcaklık stresi ve yem kısıtla-masının rat karaciğer dokusunda bazı küçük ısı şoku protein (sHSP) genlerinin mRNA düzeyindeki ekspresyonu düzeyleri üzerine etkisi-nin araştırılmasıdır. Gereç ve Yöntem: Bu amaçla on haftalık yaştaki toplam 24 Spra-gue-Dawley rat 4 gruba ayrıldı. Grup I ve Grup II'deki ratlar 22°C'lik ortam sıcaklığında, Grup III ve Grup IV'teki ratlar ise 38°C'lik ortam sıcaklığında tutuldu. Grup I ve III'teki ratlar ad libitum olarak beslen-di, Grup II ve IV'teki ratlara ise ad libitum grupların tükettiği yemin %60'ı kadar yem verildi. Uygulama 9 hafta sürdürüldükten sonra karaciğer doku örnekleri alınarak sıvı azot içerisinde donduruldu ve RNA izolasyonuna kadar muhafaza edildi. Doku örneklerinden total RNA izole edildikten sonra HspB1, HspB5, HspB6, Hsp10 ve Hsp11 genlerinin ekspresyon düzeyleri gerçek zamanlı nicel polimeraz zin-cir reaksiyonu (RT-qPCR) yöntemi ile incelendi.Bulgular: Sıcaklık stresi HspB2, HspB8 ve Hsp70 genlerinin ekspres-yonunu önemli ölçüde arttığı, HspB1, HspB5, HspB6, Hsp10 ve Hsp11 genlerinin ekspresyonunu ise etkilemediği belirlendi. Yem kısıtla-ması HspB6 geninin expresyonunu arttırırken HspB1, HspB2, HspB5, HspB8 HspB10, HspB11 ve Hsp70 genlerinin ekspresyonunu etkile-mediği gözlendi. Uygulamalar arasında interaksiyon gözlenmedi.Öneri: Çalışmanın sonuçları uzun süreli sıcaklık stresinin rat kara-ciğer dokusundaki sHSP genlerinin ekspresyonlarını değişik düzey-lerde etkilediğini, yem kısıtlamasının sHSP genlerinin sıcaklık stresi tarafından etkilenen ekspresyonlarını değştirmediğini göstermiştir.

Uzun süreli sıcaklık stresi ve yem kısıtlamasının rat karaciğer doku-sundaki bazı küçük sıcaklık şoku protein (sHSP) genlerinin ekspres-yon düzeyleri üzerine etkisi.

Aim: Investigation of the effects of dietary restriction on expression of certain small heat shock protein (sHSP) genes at mRNA level in liver tissue of rats reared under long-term heat stress. Material and Method: Sprague-Dawley rats (n=24) 10 weeks of age, were equally divided into four groups. Group I and II were kept at an ambient temperature of 22°C, while Groups III and IV were reared at 38°C. Groups I and III were fed ad libitum, while Groups II and IV were fed 60% of the diet consumed by their ad libitum counterparts. The treatment continued for 9 weeks. At the end of the treatment, liver tissue samples were taken. Total RNA was isolated and mRNA expression level of the HspB1, HspB2, HspB5, HspB6, HspB8, Hsp10, Hsp11 and HspA1A genes were assessed by Real-Time PCR analysis. Results: Heat stress significantly up regulated mRNA expressions of HspB2, HspB8 and Hsp70 genes, while it did not change mRNA exp-ressions of HspB1, HspB5, HspB6, Hsp10 and Hsp11 genes. Dietary restriction (DR) did not significantly affect the expression of HspB1, HspB2, HspB8 HspB10, HspB11 and Hsp70, while it increased mRNA expression of HspB6 gene. No interaction between treatments was observed.Conclusions: The results suggested that long term heat stress diffe-rentially affected the sHSP genes studied and DR had no affect on the heat stress mediated changes in the expression of sHSP.

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