Basal medium eagle solution may improve the post-thaw parameters of Kangal dog semen

Amaç: Bu çalışmada dondurma-çözdürme sonrası Kangal köpeği spermatolojik parametreleri üzerine BME'nin etkisi-nin ortaya konulması amaçlandı.Gereç ve Yöntem: Ejakülatlar hafta iki kez digital maniplas-yon yardımıyla alındı. Alınan ejakülatlar 6 eşit hacme bölü-nerek temel Tris sulandırıcısı içeren (T), %5 gliserol (TG), %5 etilen glikol (TE), %5 gliserol (G) + %2.5 BME (TGB2.5), TG + %10 BME (TGB10), %5 etilen glikol (E) + %2.5 BME (TEB2.5) ve TE + %10 BME (TEB10) sulandırıcılar ile su-landırıldı. Sulandırılan spermalar 4°C'ta 1.5 saat ekilibras-yon sonrası sıvı azot buharında dondurularak sıvı azotta (~-196°C) saklandı. Bulgular: Çalışmada progresif motilite oranı %10 BME içe-ren gliserollü sulandırıcı grubunda (%23.19), %5 etilen gli-kol içeren (%14.08) grubuna göre istatistiksel olarak yüksek bulundu. Akrozom bütünlüğü %2.5 BME içeren gliserollü sulandırıcı (%44.99) grubunda, %5 gliserol içeren (%33.63) grubuna göre istatiksel olarak üstünlük gösterdi (P

Amino asit sıvısı Kangal köpeği spermasının çözüm sonu spermatolojik parametrelerini iyileştirebilir

Aim: The aim of this study was to investigate the effects of BME on Kangal dog sperm parameters following the freeze-thawing process.Materials and Methods: Ejaculates were collected by digital manipulation twice a week. Ejaculates divided into six equ-al aliquots, and diluted with Tris-based extender (T) conta-ining 5% glycerol (G) + BME 2.5% (TGB2.5), TG + BME 10% (TGB10), 5% ethylene glycol (E) + BME 2.5% (TEB2.5), TE + BME 10% (TEB10), TG and TE. Subsequently, the ejaculates were cooled to 4°C at 1.5 hours, and stored in liquid nitrogen (~-196°C).Results: The extender supplemented with 10% BME + 5% G resulted in higher progressive motility (23.19%), in compa-rison to the extenders supplemented with 5% EG (14.08%). Better sperm acrosome integrity was achieved with the use of the extender containing 2.5% BME + 5% G (44.99%), when compared to the use of the extender supplemented with 5% G (33.63%) (P

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