Meriç Lütfi AVSEVER, Tansel TANRIKUL, Behire Işıl DİDİNEN, Ertan Emek ONUK

Vagococcus salmoninarum, a causative agent of disease in rainbow trout ( Oncorhynchus mykiss, Walbaum) broodstocks in the aegean region of Turkey

Türkiyenin Ege Bölgesinde yer alan ticari bir alabalık çiftliğinde gökkuşağı alabalığı anaçlarında görülen birhastalık esnasında ortalama %10 oranında bir ölüm kaydedildi. Hastalık belirtileri yumurtlama sonrası 10-12°C susıcaklığında görüldü. Klinik olarak enfekte 2 anaç balıktan 6 adet bakteriyel izolat elde edildi. Bu izolatların Gr (+) kokobasil oldukları tespit edildi ve fenotipik olarak Vagococcus salmoninarum olarak teşhis edildi. İzolatlar, aynı zamanda spesifik V. salmoninarum primerleri ile PCR metodu kullanılarak teyit edildi. VG3 suşunun antibiyotik duyarlılığıKirby-Bauer disk difüzyon metodu ile belirlendi ve amoksisillin, ampisillin, enrofoksasin, norfoksasin, oksolinik asitve forfenikole duyarlılık gösterdi. Türkiyede farklı coğrafik bölgelerden izole edilen V. salmoninarum izolatlarınınERIC-PCR sonucuna göre ilişkili olduğu (0.73) görüldü.

Türkiye nin Ege Bölgesindeki gökkuşağı alabalığı (Oncorhynchus mykiss, Walbaum) anaçlarında bir hastalık etkeni Vagococcus salmoninarum

In a commercial rainbow trout farm located in the Aegean region of Turkey, a disease outbreak with an aver-age mortality of 10% was recorded in rainbow trout broodstocks. The disease symptoms emerged at 10-12°C water tem-perature after spawning periods. 6 bacterial isolates were obtained from 2 of the clinically infected rainbow trout brood-stock. These isolates were observed to be Gram-positive cocobacilli and were identifed phenotypically as Vagococcussalmoninarum . Isolates were also confrmed by using a PCR method with V.salmoninarum specifc primers. Antibioticsusceptibility of strain VG3 was assessed by the Kirby-Bauer disk diffusion method, and the results showed that it wassusceptible amoxycillin, ampicillin, enrofoxacin, norfoxacine, oxolinic acid and forfenicol. V.salmoninarum isolatesisolated from different geographical region in Turkey were revealed to be related (0.73) as a result of ERIC-PCR.

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