Tansel TANRIKUL, Meriç Lütfi AVSEVER, Ertan Emek ONUK, Behire Işıl DİDİNEN

Türkiye’nin Ege Bölgesindeki gökkuşağı alabalığı (Oncorhynchus mykiss, Walbaum) anaçlarında bir hastalık etkeni Vagococcus salmoninarum

Türkiye’nin Ege Bölgesi’nde yer alan ticari bir alabalık çiftliğinde gökkuşağı alabalığı anaçlarında görülen bir hastalık esnasında ortalama %10 oranında bir ölüm kaydedildi. Hastalık belirtileri yumurtlama sonrası 10-12°C su sıcaklığında görüldü. Klinik olarak enfekte 2 anaç balıktan 6 adet bakteriyel izolat elde edildi. Bu izolatların Gr (+) kokobasil oldukları tespit edildi ve fenotipik olarak Vagococcus salmoninarum olarak teşhis edildi. İzolatlar, aynı zamanda spesifik V. salmoninarum primerleri ile PCR metodu kullanılarak teyit edildi. VG3 suşunun antibiyotik duyarlılığı Kirby-Bauer disk difüzyon metodu ile belirlendi ve amoksisillin, ampisillin, enrofloksasin, norfloksasin, oksolinik asit ve florfenikole duyarlılık gösterdi. Türkiye’de farklı coğrafik bölgelerden izole edilen V. salmoninarum izolatlarının ERIC-PCR sonucuna göre ilişkili olduğu (0.73) görüldü.

Anahtar Kelimeler:

Tansel TANRIKUL, Meriç Lütfi AVSEVER, Ertan Emek ONUK, Behire Işıl DİDİNEN, Oncorhynchus mykiss, fenotipik, genotipik, karakterizasyon, Vagococcus salmoninarum, 2014, Etlik Veteriner Mikrobiyoloji Dergisi

Vagococcus salmoninarum, a causative agent of disease in rainbow trout (Oncorhynchus mykiss, Walbaum) broodstocks in the aegean region of Turkey

In a commercial rainbow trout farm located in the Aegean region of Turkey, a disease outbreak with an average mortality of 10% was recorded in rainbow trout broodstocks. The disease symptoms emerged at 10-12°C water temperature after spawning periods. 6 bacterial isolates were obtained from 2 of the clinically infected rainbow trout broodstock. These isolates were observed to be Gram-positive cocobacilli and were identified phenotypically as Vagococcus salmoninarum. Isolates were also confirmed by using a PCR method with V.salmoninarum specific primers. Antibiotic susceptibility of strain VG3 was assessed by the Kirby-Bauer disk diffusion method, and the results showed that it was susceptible amoxycillin, ampicillin, enrofloxacin, norfloxacine, oxolinic acid and florfenicol. V.salmoninarum isolisolates isolated from different geographical region in Turkey were revealed to be related (0.73) as a result of ERIC-PCR. Key words: Oncorhynchus mykiss, phenotypic, genotypic, characterization, Vagococcus salmoninarum.

Keywords:

Oncorhynchus mykiss, phenotypic, genotypic, characterization, Vagococcus salmoninarum,

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Altun S., Onuk EE, Çiftci A, Büyükekiz AG, Duman M, (2012). Phenotypic, Genotypic Characterization and Antimicrobial Susceptibility Determination of Lactoccous garvieae Strains. Kafkas Univ Vet Fak Derg. 19(3), 375-381.
Austin B, Austin DA (2007). Bacterial Fish Pathogens: Diseases of Farmed and Wild Fish, 4th edition, Springer- Praxis, Chichester, UK, p.552.
Beaz-Hidalgo R, Lopez-Romalde S, Toranzo AE, Romalde JL, (2008). Polymerase Chain Reaction Amplification of Repetitive Intergenic Consensus and Repetitive Extragenic Palindromic Sequences for Molecular Typing of Pseudomonas anguilliseptica and Aeromonas salmonicida. J Aqua Anim Health. 20, 75-85.
FAO. National Aquaculture Sector Overview Turkey. Fisheries and Aquaculture Department. Erişim adresi: http://www.fao.org/fishery/countrysector/naso_turkey/ en#tcN90158, Erişim tarihi:2013
Didinen BI, Kubilay A, Diler, O, Ekici S, Onuk EE, Findik A, (2011). First isolation of Vagococcus salmoninarum from cultured rainbow trout (Oncorhynchus mykiss, Walbaum) broodstocks in Turkey. Bull Eur Ass Fish Pathol. 31(6), 235-243.
Ghittino, C, Agnetti, F, Panzieri, C, Cabra, S, Colussi, S, Prearo M, (2004). L’ infezione da Vagococcus salmoninarum della trota iridea in Italia. Ittiopatologia 1, 25-33.
Harlioglu, AG, (2011). Present status of fisheries in Turkey. Rev Fish Biol Fisher. 21, 667-680.
Michel, C., Nougayrede, P., Eldar, A., Sochon, E., de Kinkelin P, (1997). Vagococcus salmoninarum, a bacterium of pathological significance in rainbow trout Oncorhynchus mykiss farming. Dis Aquat Organ. 30, 199-208.
NCCLS, (2001). National Committee for Clinical Laboratory Standards of Antimicrobial Susceptibility Testing. Performance Standards for Antimicrobial Susceptibility Testing. Eleventh Information Supplement. NCCLS document M100-S11 NCCLS, West Walley Road, Suite 1400, Wayne, Pennsylvania 19087-1898 USA.
Onuk, EE, Çiftçi, A, Fındık, A, Çiftçi, G, Altun, S, Balta, F, Ozer, S, Coban AY, (2011). Phenotypic and Molecular Characterization of Yersinia ruckeri Isolates from Rainbow Trout (Oncorhynchus mykiss, Walbaum, 1792) in Turkey. Berl Munch Tierarztl Wochensch. 124 (7-8), 320-328.
Ruiz-Zarzuela, I, de Blas, I, Girones, O, Ghittino, C, Muzquiz JL, (2005). Isolation of Vagococcus salmoninarum in rainbow trout Oncorhynchus mykiss (Walbaum), broodstocks: characterisation of the pathogen. Vet Res Commun. 29, 553-562.
Salogni, C, Perantoni, P, Pitozzi, A, Loris, G, Alborali GL, (2007). Vagococcus salmoninarum: descrizione di un focolaio di malattia in riproduttori di trota iridea (Oncorhynchus mykiss). Ittiopatologia 4, 59-66.
Schmidtke, LN and Carson J, (1994). Characteristics of Vagococcus salmoninarum isolated from diseased salmonid fish. J Appl Bacteriol. 77, 229-236.
Thrush, MA, Dunn, PL, Peeler EJ, (2012). Monitoring Emerging Diseases of Fish and Shellﬁsh Using Electronic Sources. Transbound Emerg Dis. 59, 385-394.
Toranzo, AE, Magarinos, B., Romalde JL, (2005). A review of the main bacterial fish diseases in mariculture systems. Aquaculture 246, 37-61.
Versalovic, J, Koeuth, T, Lupski R, (1991). Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Res. 19(24), 6823-6831.
Yılmaz, S, Akay, AS, Gumus E, (2008). Fisheries sector in Turkish economy and marketing of fishery products. Akdeniz Univ. Ziraat Fak. Derg. 21, 265-272.