A.ebru BORUM, Sertan ÇATIK, Zafer MECİTOĞLU, Gülşah DEMİR, Mihriban ÜLGEN, Sezgin ŞENTÜRK

ELISA ve fekal bakteriyoskopi ile sığırlarda Paratuberküloz prevalansının belirlenmesi

Bu çalışmada Afyon ve civarındaki çiftliklerden 4-8 yaş arasındaki 305 Holstein Fresian sütçü sığırlar kullanılmıştır. Bu sığırların iki tanesinde kronik diare şikayeti mevcuttu ve yapılan tedaviye cevap vermemiştir. Sürüdeki diğer sığırlarda klinik inceleme yapılmış, hematolojik parametrelere bakılmış, teşhis amaçlı dışkı ve kan serumu örnekleri alınmıştır. Mycobacterium avium subsp. paratuberculosis (MAP) serum örneklerinde ELISA yöntemi kullanılarak tespit edildi. Süt sığırlannda paratuberküloz’un prevalansı, dışkı örneklerinde; Ziehl-Neelsen boyama ile %4.59, ELISA testi ile ise %31.80 oranında pozitif tespit edilmiştir

Anahtar Kelimeler:

Sezgin ŞENTÜRK, Mihriban ÜLGEN, Zafer MECİTOĞLU, Sertan ÇATIK, Gülşah DEMİR, A.Ebru BORUM, ELISA, Paratüberküloz, Ziehl Neelsen Boyama, 2014, Etlik Veteriner Mikrobiyoloji Dergisi

Detection of bovine Partuberculosis with ELISA and fecal examination

In this study, 305 between 4-8 years on a farm in Holstein Fresian dairy cows in Afyon were used. In two of these animals with chronic diarrhea were present and cows did not respond to treatment. Clinical studies, hematological parameters were determined, and blood serum and fecal samples were taken in other cattle in the herd. Mycobacterium avium subsp. Paratuberculosis (MAP) in serum samples was determined using ELISA. The prevalence of paratuberculosis in dairy cows, Ziehl-Neelsen staining with 4.59% in fecal samples, 31.80% positive by the ELISA test were determined.

Keywords:

ELISA, Paratuberculosis, Ziehl Neelsen Staining,

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