Öz In this study, we investigated the potential roles of BDV and Brucella melitensis infections in a case of sheep abortion. Internal organ specimens from aborted sheep foetus and EDTA whole blood sample from mother of the foetus were collected from a sheep flock in the Konya Province in the Central Anatolia region of Turkey in 2017. The presence of Brucella spp. directly assessed by bacterial isolation and detection of BDV was carried by real time RT-PCR. Genetic characterization of the BDV field isolate was conducted by sequencing the 5’- end untranslated region (UTR) region of BDV. Brucella strain was isolated from the samples of aborted sheep foetus, and it was identified as Brucella melitensis by biochemical characteristics, agglutination with monospecific A and M sera. BDV RNA was detected in EDTA whole blood sample and aborted sheep foetus. Phylogenetic analysis in 5’-UTR region allocated the field isolate of BDV obtained in this study into BDV-7 genotype. To the best of our knowledge, this is the first report on the dual infection of aborted sheep foetus with BDV and Brucella melitensis.
1. Becher P, Orlich M, Shannon AD, Horner G, König M, Thiel HJ, (1997). Phylogenetic analysis of pestiviruses from domestic and wild ruminants. J Gen Virol. 78, 1357-1366.
2. Cabezón O, Rosell R, Velarde R, Mentaberre G, Casas-Díaz E, Lavín S, Marco I, (2010). Border disease virus shedding and detection in naturally infected Pyrenean chamois (Rupicapra pyrenaica). J Vet Diagn Invest. 22, 744-747.
3. Díaz Aparicio E, (2013). Epidemiology of brucellosis in domestic animals caused by Brucella melitensis, Brucella suis and Brucella abortus. Rev Sci Tech. 32, 43-51, 53-60.
4. European Commission, Health & Consumers Directorate- General (2012). Eradication programme for Sheep and Goat Brucellosis (B. Melitensis). Erişim adresi: https:// ec.europa.eu/food/sites/food/files/safety/docs/cff_animal_ vet-progs_2012_dec-2011-807-ec_ov-cap-brucellosis_grc. pdf, Erişim tarihi: 08.08.2017
5. Hawari AD, (2012). Epidemiological Studies, Seroprevalance and Some Risk Factors of Brucellosis in Sheep and Goats in the South Province of West Bank. Asian J Anim Vet Adv. 7, 535-539.
6. Heinz FX, Collett MS, Purcell RH, Gould EA, Howard CR, Houghton M, Moormann RJM, Rice CM, Tiehl HJ, (2000). Family Flaviviridae, Virus Taxonomy. Seventh Report of the International Committee on Taxonomy of Viruses. San Diego: Academic Pres, p. 859-878.
7. La Rocca SA, Sandvik T, (2009). A short target real-time RT-PCR assay for detection of pestiviruses infecting cattle. J Virol Methods. 161, 122-127.
8. Marco I, Lopez-Olvera JR, Rosell R, Vidal E, Hurtado A, Juste R, Pumarola M, Lavin S, (2007). Severe outbreak of disease in the southern chamois (Rupicapra pyrenaica) associated with border disease virus infection. Vet Microbiol. 120, 33-41.
9. Nettleton PF, Gilray JA, Russo P, Dlissi E, (1998). Border disease of sheep and goats. Vet Res. 29, 327-340.
10. Oguzoglu TC, Tan MT, Toplu N, Demir AB, Bilge-Dagalp S, Karaoglu T, Ozkul A, Alkan F, Burgu I, Haas L, Greiser- Wilke I, (2009). Border disease virus (BDV) infections of small ruminants in Turkey: a new BDV subgroup? Vet Microbiol. 135, 374-379.
11. Vilcek S, Herring AJ, Herring JA, Nettleton PF, Lowings JP, Paton DJ, (1994). Pestiviruses isolated from pigs, cattle and sheep can be allocated into at least three genogroups using polymerase chain reaction and restriction endonuclease analysis. Arch Virol. 136, 309-323.
12. Office International des Epizooties (OIE), (2017). Border Disease. In Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. OIE, Paris, 1-13. Available at: http:// www.oie.int/fileadmin/Home/eng/Health_standards/ tahm/2.07.01_BORDER_DIS.pdf (accessed on 25 August 2017).