Dual Infection of Sheep Aborted Foetus with Peste des Petits Ruminants Virus and Brucella melitensis

In this study, we investigated the potential roles of Brucella melitensis and PPR virus (PPRV) infections in a case of sheep abortion. Samples were collected from PPR-suspected ewe and its aborted foetus from a sheep flock in the Antalya Province in the Mediterranean region of Turkey in 2016. The presence of Brucella spp. directly assessed by bacterial isolation and detection of PPRV was carried by real time RT-PCR. Genetic characterization of the PPRV field isolates was conducted by sequencing the fusion (F) gene of PPRV. Brucella strain was isolated from the samples of aborted sheep foetus, and it was identified as Brucella melitensis by biochemical characteristics, agglutination with monospecific A and M sera. PPRV RNA was detected in samples of PPR-suspected ewe and its foetus. Phylogenetic analysis showed that the field isolate of PPRV obtained in this study was clustered within lineage IV. To the best of our knowledge, this is the first report on the dual infection of aborted sheep foetus with PPRV and Brucella melitensis.

Anahtar Kelimeler:

Sheep foetus, Peste des petits ruminants virus, Genetic characterization, F gene, Brucella melitensis

PDF

___

1. Batten CA, Banyard AC, King DP, Henstock MR, Edwards L, Sanders A, Buczkowski H, Oura CC, Barrett T, (2011). A real time RT-PCR assay for the specific detection of Peste des petits ruminants virus. J Virol Methods. 171, 401-404.
2. Büyükcangaz E, Şen A, Kahya S, (2009). Isolation and biotyping of Brucella melitensis from aborted sheep and goat foetuses. Turk. J Vet Anim Sci. 33, 311-316.
3. Couacy-Hymann E, Bodjo SC, Koffi MY, Kouakou C, Danho T, (2009). The early detection of peste-des-petits-ruminants (PPR) virus antigens and nu-cleic acid from experimentally infected goats using RT-PCR and immunocapture ELISA techniques. Res Vet Sci. 87, 332-335.
4. European Commission, Health & Consumers Directorate- General (2012). Eradication programme for Sheep and Goat Brucellosis (B. Melitensis). Erişim adresi: https:// ec.europa.eu/food/sites/food/files/safety/docs/cff_animal_ vet-progs_2012_dec-2011-807-ec_ov-cap-brucellosis_grc. pdf, Erişim tarihi: 08.08.2017
5. Forsyth MA, Barrett T, (1995). Evaluation of polymerase chain reaction for the detection and characterisation of rinderpest and peste des petits ruminants viruses for epidemiological studies. Virus Res. 39, 151-163.
6. Gibbs PJE, Taylor WP, Lawman MP, Bryant J, (1979). Classification of the peste des petits ruminants virus as the fourth member of the genus Morbillivirus. Intervirology 11, 268-274.
7. Hawari AD, (2012). Epidemiological Studies, Seroprevalance and Some Risk Factors of Brucellosis in Sheep and Goats in the South Province of West Bank. Asian J Anim Vet Adv. 7, 535-539.
8. Ilhan Z, Solmaz H, Aksakal A, Gülhan T, Ekin IH, Boynukara B, (2007). Comparison of PCR assay and bacteriological culture method for the detection of Brucella melitensis in stomach content samples of aborted sheep fetuses. Dtsch Tierarztl Wochenschr. 114, 460-464.
9. Kul O, Kabakci N, Ozkul A, Kalender H, Atmaca HT, (2008). Concurrent peste des petits ruminants virus and pestivirus infection in stillborn twin lambs. Vet Pathol. 45, 191-196.
10. La Rocca SA, Sandvik T, (2009). A short target real-time RT-PCR assay for detection of pestiviruses infecting cattle. J Virol Methods. 161, 122-127.
11. Office International des Epizooties (OIE) (2016). Brucellosis (Brucella abortus, B. melitensis and B. suis). Erişim adresi: http://www.oie.int/fileadmin/Home/eng/Health_standards/ tahm/2.01.04_BRUCELLOSIS.pdf, Erişim tarihi: 24.10.2017
12. Office International des Epizooties (OIE) (2017). Border Disease. Erişim adresi: http://www.oie.int/fileadmin/Home/ eng/Health_standards/tahm/2.07.01_BORDER_DIS.pdf, Erişim tarihi: 24.10.2017
13. Ozkul A, Akca Y, Alkan F, Barrett T, Karaoglu T, Dagalp SB, Anderson J, Yesilbag K, Cokcaliskan C, Gencay A, Burgu I, (2002). Prevalence, distribution, and host range of peste des petits ruminants virus, Turkey. Emerg Infect Dis. 8, 708-712.
14. Pappas G, Akritidis N, Bosilkovski M, Tsianos E, (2005). Medical progress Brucellosis. N Engl J Med. 352, 2325- 2367.
15. Şevik M, (2014). Molecular Detection of Peste des Petits Ruminants Virus from Different Organs/Tissues of Naturally Infected Animals. Kafkas Univ Vet Fak Derg. 20, 165-168. 16. Şevik M, Sait A, (2015). Genetic characterization of peste des petits ruminants virus, Turkey, 2009-2013. Res Vet Sci. 101, 187-195.