Borrelia burgdorferi ile doğal enfekte köpeklerin nötrofil-lenfosit, monosit-lenfosit ve platelet-lenfosit oranlarının belirlenmesi

Lyme hastalığı ya da Borreliozis; Borrelia burgdorferi adlı spiroket şekilli bir bakterinin neden olduğu, dünyada köpeklerde ve insanlarda yaygın olarak görülen, başlıca Ixodes cinsi kenelerle bulaşan, kalp, eklemler ve sinir sisteminde bozukluklarla karakterize ve kronikleşebilen enfeksiyöz bir hastalıktır. Günümüzde sistemik inflamatuar yanıtın komplementleri olarak nötrofil-lenfosit oranı (NLO), monosit-lenfosit oranı (MLO) ve platalet-lenfosit oranı (PLO) birçok hastalık prognozunun değerlendirilmesi ve prediktif parametreler olarak kullanılmaktadır. Bu çalışmadaki amacımız ise ilk defa kronik bir inflamasyona neden olan B. burgdorferi’i yönünden doğal enfekte seropozitif köpekler ile seronegatif köpeklerin tedavi öncesi başüstü parametreleri olan nötrofil (NEU), NLO, lenfosit (LYM), monosit (MONO), MLO, platalet (PLT) ve PLO değerlerinin karşılaştırılmasıdır. Yirmi dört adet sahipli köpekten tedavi öncesi alınan tam kan ve serum örnekleri çalışma materyalini oluşturmuştur. Serum örneklerinin B. burgdorferi’ye karşı oluşan IgG antikor yanıtı ELISA yöntemi ile belirlenmiştir. Sonrasında bu 7 adet seropozitif örnek Western Blot yöntemi ile doğrulanmıştır. Gruplar arası NEU, NLO, MONO, MLO, PLT ve PLO değerleri karşılaştırılmış ancak, istatiksel bir fark bulunamamıştır. Sonuç olarak lyme hastalığı yönünden seropozitif köpekler ile seronegatif köpeklerin ilk kez NLO, MLO, ve PLO değerlerinin karşılaştırıldığı mevcut çalışma ile uygulaması kolay ve düşük maliyetli sistemik inflamasyon belirteçlerinin veteriner pratikteki kullanımlarının genişletilmesi yönündeki gerçekleştirilecek çalışmalara katkı sağlayacağı düşünülmektedir.

Anahtar Kelimeler:

Köpek, Borrelia burgdorferi, nötrofil-lenfosit, monosit-lenfosit, platelet-lenfosit

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