Özge ÖZBAHAR, Gökçen Yuvalı ÇELİK, İsmail ÖÇSOY, Dilşad ONBAŞLI

Pisolithus arrhizus Ekstraktı Kullanılarak Sentezlenen Titanyum Nanopartikülünün Antimikrobiyal Etkisi

Pisolithus arrhizus (Scop.) Rauschert (Basidiomycetes) türü okaliptüs ve çam ağaçlarının birçok türünü çevreleyen kökleri toprakta bulunan bir ektomikorizal mantardır. Bu araştırmada Pisolithus arrhizus etanol ekstraktı kullanılarak hazırlanan Titanyum (TiO2) nanopartikülünün (NP) farklı patojen mikroorganizmalar üzerinde inhibitörik etkisinin tespit edilmesi amaçlanmıştır. Çalışmada hydrothermal yöntem kullanılarak sentezlenen mantar ekstreli TiO2 NP, UV-Vis Spektrometre (UV-vis) ve taramalı elektron mikroskop (SEM) ile karakterize edilmiştir. Hazırlanan ve karakterize edilen bu NP’lerin, disk difüzyon yöntemi kullanılarak Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Candida albicans ve Streptococcus mutans test patojenleri üzerinde antimikrobiyal aktivitesi araştırılmıştır. Mantar ekstreli TiO2 NP’lerin patojenler üzerinde zayıf bir inhibisyon etkisine sahip oldukları (7.5-9.3 mm), maksimum inhibisyonu (9.3 mm) S. mutans'a karşı gösterdiği buna karşın C. albicans’a karşı antifungal etkiye sahip olmadıkları tespit edilmiştir.

Anahtar Kelimeler:

Antimikrobiyal aktivite, Pisolithus arrhizus, TiO2 nanopartikülü

Antimicrobial Effect of Titanium Nanoparticles Synthesized Using Pisolithus arrhizus Extraction

Pisolithus arrhizus (Scop.) Rauschert (Basidiomycetes) type is an ectomycorrhizal fungi found in the soil that surrounds many species of eucalyptus and pine trees. In this study, it was aimed to determine the inhibitory effect of Titanium (TiO2) nanoparticle (NP) prepared by using Pisolithus arrhizus ethanol extract on different pathogenic microorganisms. In the study, NP’s synthesized using hydrothermal method was characterized by UV-vis spectrometer (UV-vis) and scanning electron microscope (SEM). The antimicrobial activity of these prepared and characterized NP’s, have been investigated by using disk diffusion method on test pathogens of Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Candida albicans and Streptococcus mutans. While NPs have a weak inhibitory effect on pathogens (7.5-9.3 mm), the maximum inhibition (9.3 mm) is shown against S. mutans. Whereas antifungal effect against C. albicans have not been found.

Keywords:

Antimicrobial activity, Pisolithus arrhizus, TiO2 nanoparticle,

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