ELİF BAYSAL, SİBEL OĞUZKAN BALCI, Fatih ÇELENK, Merve KAHRAMAN, Murat DENİZ, ORHAN TUNÇ, Cengiz DURUCU, LÜTFİ SEMİH MUMBUÇ, MUZAFFER KANLIKAMA, SACİDE PEHLİVAN

Sitokin geni polimorfizmleri ve Türk pediyatrik koklear implant hastalar>ndaki ekspresyonu

Amaç: Çalıflmamızda polimorfizmler, üç adet sitokin geni ekspresyonu ve konjenital sensorinöral iflitme kaybı tedavisi için koklear implantasyon uygulanmıfl çocukların klinik parametreleri arasındaki iliflkiyi inceledik. Yöntem:IL-6/-174, IFN-?/+874 ve TNF-?/-308 genleri, 64 konjenital sensorinöral iflitme kaybı hastasında ve 70 sağlıklı kontrol olgusunda analiz edildi. Sitokin ekspresyonu PCR-SSP yöntemi ile gerçeklefltirilmifltir. Bulgular:Hasta grubu ve sağlıklı kontrol grubu arasında TNF-? veya IL-6'nın genotipleri ve allel frekansları sayısı ve dağılımı bakımından belirgin bir farklılık saptanmadı. Ancak yüksek IFN-? ekspresyonu ile iliflkilendirilen TT genotipi ve T allel frekansı, hasta grubunda kontrol grubuna göre daha sıklıkla gözlendi (sırasıyla p=0.016 vep=0.023). Sonuç:Sonuçlarımız yüksek oranda IFN-? geni ekspresyonunun hastalığa olan duyarlılığı arttırdığını göstermifltir. Bunu takiben IFN-?geninin konjenital iflitme kaybının etyopatogenezinde yararlı bir belirteç olabileceği sonucu ortaya çıkmıfltır.

Cytokine gene polymorphisms and expression in Turkish pediatric cochlear implant patients

Objective:We assessed the association between the polymorphismsand expressions of three cytokine genes and clinical parameters inchildren who underwent cochlear implantation due to profound congenital sensorineural hearing loss. Methods:We analyzed the IL-6/-174, IFN-?/+874 and TNF-?/308 genes in 64 cases with congenital sensorineural hearing loss andin 70 healthy controls. Cytokine genotyping/expression was performed using the PCR-SSP method. Results: No significant differences were detected between the patientgroup and the healthy controls with respect to the distributions andnumbers of genotypes and alleles of TNF-? or IL-6. However, theTT genotype, associated with high expression of IFN-?, and the Tallele frequency were significantly more frequent in the patient groupversus the controls (p=0.016 and 0.023, respectively). Conclusion:Our results suggest that high expression of the IFN-?gene may be associated with susceptibility to the disease.Consequently, IFN-? may be a useful marker of the etiopathogenesisof congenital sensorineural hearing loss.

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