Eskişehir ili kabakgil ekim alanlarında görülen virüs hastalıklarının belirlenmesi

Amaç: Bu araştırma, Eskişehir ili kabakgil ekim alanlarında virüs enfeksiyonlarını ve bazı yerel çerezlik kabak genotiplerinin Zucchini yellow mosaic potyvirus (ZYMV) ve Watermelon mosaic potyvirus (WMV)’ye reaksiyonlarının belirlenmesi amacıyla yapılmıştır. Materyal ve Metod: 2019 yılı Ağustos-Eylül aylarında Eskişehir ili kabakgil ekim alanlarında gerçekleştirilen surveyler sonucunda toplanan 164 adet bitki örneği DAS-ELISA yöntemi ile ZYMV, WMV, Cucurbit aphid borne yellows polerovirus (CABYV), Cucumber mosaic cucumovirus (CMV), Papaya ringspot potyvirus-watermelon (PRSV-W), Cucurbit green mottle mosaic virus (CGMMV) ve Squash mosaic comovirus (SqMV) etmenlerine karşı testlenmişlerdir. Bulgular: DAS-ELISA sonuçlarına göre, kabakgil bitkilerinde CABYV (%49.35), WMV (%44.87), ZYMV (%24.35), CMV (%17.30), PRSV-W (%1.28) ve SqMV (%1.92) ve yabancı ot örneklerinde ise ZYMV (%12.5) ve CABYV (%12.5) yaygın virüslerdir. Çerezlik kabak bitkilerinde mekanik inokulasyon çalışmalarının sonucuna göre, WMV’nin 5, 8 ve 9 numaralı genotipleri (%100) ve ZYMV’nin ise 9 (%95), 3 (%93) ve 7 (%91) numaralı genotipleri yüksek oranda hastalandırdığı belirlenmiştir. Eskişehir ilinde virüs hastalıklarının ortalama yaygınlık oranı %4.13 olarak belirlenmiştir. Sonuç: Eskişehir ilinde yapılan bu çalışma ile kabakgil üretimi yapılan alanlarda sorun olan virüs etmenleri ilk kez DAS-ELISA ile ortaya konulmuştur.

Anahtar Kelimeler:

CABYV, DAS-ELISA, Mekanik inokulasyon, Virüs, WMV, ZYMV

Detection of virus diseases in cucurbit growing areas in Eskişehir province

Objective: This research was conducted to detection of potential virus infections and to determine reactions of some local edible seed squash genotypes to Watermelon mosaic potyvirus (WMV) and Zucchini yellow mosaic potyvirus (ZYMV) in cucurbit cultivation areas of Eskişehir province. Material and Methods: As a result of the survey performed in the cucurbit cultivated fields of Eskişehir province during August to September in 2019, a total of 164 plant samples were collected and they were tested for infections of ZYMV, WMV, Cucurbit aphid-borne yellows polerovirus (CABYV), Cucumber mosaic cucumovirus (CMV), Papaya ringspot potyvirus-watermelon (PRSV-W), Cucurbit green mottle mosaic virus (CGMMV) and Squash mosaic comovirus (SqMV) with DAS-ELISA method. Results: According to DAS-ELISA results, CABYV (49.35%), WMV (44.87%), ZYMV (24.35%), CMV (17.30%), PRSV-W (1.28%) and SqMV (1.92%) were detected the most prevalent viruses in cucurbits samples, respectively while ZYMV (12.5%) and CABYV (12.5%) were found most common virus pathogens in weed samples. As to the results of mechanical inoculation studies with edible seed squash plants, WMV highly infected-genotypes were 5, 8, and 9 (100%), while ZYMV highly infected-genotypes were determined as 9 (95%), 3 (93%), and 7 (91%). The average prevalence rate of virus diseases in Eskişehir was determined as 4.13%. Conclusion: With this study conducted in Eskişehir province, viruses cause serious diseases in cucurbit growing areas were revealed for the first time by DAS-ELISA method.

Keywords:

CABYV, DAS-ELISA, Mechanical inoculation, Virus, WMV, ZYMV,

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