Meltem EVİRGAN, Deniz İŞTİPLİLER, Muzaffer TOSUN, Hülya YILMAZ TEMEL, Ferit TURANLI, Fatma AYKUT TONK

Ekmeklik buğdayda (Triticum aestivum L.) Rus buğday afidine dayanıklılık geninin ayrıntılı genetik haritalaması

Amaç: Bu çalışmanın amacı Rus buğday afidine dayanıklılık sağlayan Dn100695 geninin bulunduğu 7D kromozomunu SSR markörleriyle ayrıntılı bir şekilde taramak ve dayanıklılık genini çevreleyen yeni SSR markörlerini belirlemektir. Materyal ve Yöntem: Basribey x IG 100695 melezinin 190 bireyinden oluşan F2 populasyonu haritalama amacıyla kullanılmıştır. Haritalama populasyonu daha önceki markörlere ilave olarak 7D kromozomunda bulunan 20 yeni SSR markörleri ile taranmıştır. Zararlının populasyondaki kloroz, kıvrılma ve afit yoğunluğu özellikleri fenotipik veriler olarak ele alınmıştır. Araştırma Bulguları ve Sonuç: Çalışma sonucunda 85 polimorfik markör içerisinden 46’sı toplamda 1226.9 cM’lık bir genom bölgesini haritalandırmış ve 14 farklı bağlantı grubu oluşturmuştur. 7D kromozomu üzerinde dayanıklılık genini çevreleyen özgün bir SSR markörü (GWM121) belirlenmiş ve Dn100695 genini her iki yönden çevreleyen markörler haritalanmıştır. Ayrıca markör regresyon analizi sonucunda Rus buğday afidi dayanıklılığına katkı sağlama potansiyeli bulunan SSR markörleri de saptanmıştır.

Anahtar Kelimeler:

Buğday, Rus buğday afidi, Diuraphis noxia, SSR, Ayrıntılı haritalama

Fine Mapping of Russian Wheat Aphid Resistance Gene in Bread Wheat (Triticum aestivum L.)

Objective: The aim of this study was to screen the chromosome 7D with SSR markers and to identify unique markers surrounding the Russian wheat aphid resistance gene Dn100695. Material and methods: The F2 population consisting of 190 individuals of the Basribey x IG 100695 cross was used for mapping. The mapping population was screened with 20 SSR markers located on the 7D chromosome in addition to the previous markers. Chlorosis, rolling and aphid density characteristics of the pest in the population were considered as phenotypic data. Results and conclusion: As a result of the study, 46 of the 85 polymorphic markers mapped a genome region of 1226.9 cM in total and formed 14 different linkage groups. A unique SSR marker (GWM121) flanking the resistance gene was identified on the 7D chromosome, and markers flanking the Dn100695 gene from both directions were mapped. Additionally, as a result of the marker regression analysis, SSR markers that have the potential to contribute to Russian wheat aphid resistance were determined.

Keywords:

Wheat, Russian wheat aphid, Diuraphis noxia, SSR, Fine mapping,

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