Giray Nazan YALAZ UYSAl, Osman AÇIKIGÖZ, Sevil GÖNENÇ, Muammer KAYATEKİN

Sıçanlarda L-karnitinin streptozotocinle diyabet oluşumu ve oksidan stres üzerine etkilerinin araştırılması

Amaç: Bu çakşmanın amacı L-karnitinin diyabeti önlemedeki rolünün ve pankreas lipid peroksidasyonu üzerine etkilerinin incelenmesidir. Gereç ve Yöntem: Çalışmada erişkin Wister Albino cinsi erkek sıçanlar kullanıldı. Deneysel diyabet oluşturmak amacıyla 65 mg/kg İP streptozotocin uygulandı. SOD, GPx ve glukoz ölçümleri spektrofotometrik yöntemle yapıldı. Protein miktarları Markwell'in modifiye lowry MDA değerleri ise Rehnchona ve ark.yönternine göre ölçüldü. Bulgular: Streptozotocinden önce L-karnitin verilmesinin streptozotocinle oluşan diyabette kan şekeri üzerine bir etkisi olmamıştır. Pankreas süperoksit dismutaz enzim aktivitesinde anlamlı bir farklılık görülmemiştir ancak glutatyon peroksidaz (GPx) enzim aktivitesi diyabetik grupta 2 g/kg karnitin alan gruba ve 500 mg/kg karnitinalan diyabetik gruba göre yüksek olarak bulunmuştur. Sonuç: L-karnitinin streptozotocinle oluşan diyabet üzerine bir etkisi görülmemiştir.

Anahtar Kelimeler:

Karnitin, Diabetes mellitus, Lipid peroksidasyonu, Oksidatif stres, Modeller, teorik, Modeller, hayvan, Sıçan, Wistar, Streptozosin

The effects of L-carnitine on streptozotocin inducerd diabetes mellitus and oxidative stress

Objective: The aim of this study was to investigate the role of L-carnitine on preventing diabetes nd its effects on pancreas lipid peroxidation. Material and Methods: Adult Wistar Albino male rats were used in this study. Single dose 65 mg/kg IP. streptozotocin enjected to onset experimental diabetes mellitus. SOD, GPx and glucose measurements made by spectrophotometric methods. Protein levels were measured by Markwell's modified Lowry method and MDA levels were measured by the method of Rehnchona et al. Results: Giving L-carnitine prior to treptozotocin did not have any effects on the blood glucose levels in diabetes induced by treptozotocin. There was no significant change on the pancreas süperokside dismutase enzyme activity and TEARS levels, however glutathione peroksidase (GPx) activity was found to be increased in the diabetic group and compared the carnitin-taking group and the carnitin-taking diabetic group. Conclusion: No effect of L-carnitine was observed on streptozotocin induced diabetes.

Keywords:

Carnitine, Diabetes Mellitus, Lipid Peroxidation, Oxidative Stress, Models, Theoretical, Models, Animal, Rats, Wistar, Streptozocin,

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