KEMAL KÜRŞAD GENÇ, ŞERMİN GENÇ, Ülker SÖNMEZ, Osman YILMAZ, Ataç SÖNMEZ, Kazım TUĞYAN, Bakir ERGÜR, Zişan BULDAN

Primer oligodendrosit kültüründe metamfetamine bağlı hücre ölümü

Amaç: Psikostimülan bir ilaç olan metamfetaminin nörotoksik etkisi in vitro ve in vivo çalışmalarla kanıtlanmıştır. Bu çalışmada ise metamfetaminin yenidoğan Wistar sıçan oligodendrosit kültürlerinde sitotoksik etkisi araştırıldı. Gereç ve Yöntem: Metamfetaminin sitotoksik etkisi laktat dehidrogenaz (LDH) testiyle, apoptoz indükleyici etkisi ise apostain immünfloresan boyama yöntemiyle araştırıldı. Bu amaçla kültürler değişen konsantrasyonlarda (10-1000 $\mu$M arası) ve değişik enkübasyon sürelerinde (24-96 saat arası) metamfetamiııe maruz bırakıldı. Bulgular: LDH ölçümleri metamfetaminin doza ve zamana bağımlı olarak oligodendrosit ölümüne yol açtığını göstermektedir. Apostain yöntemi ise metamfetaminin düşük (10 $\mu$M) ve yüksek (1000 $\mu$M) dozlarda daha belirgin apoptoza neden olduğunu işaret etmektedir. Sonuç: Bu sonuçlar kronik metamfetamin kullanıcılarında gözlenen nörotoksik etkilerin kısmen bu maddenin oligodendrositler üzerine toksik etkisine de bağlı olabileceğini düşündürmektedir.

Anahtar Kelimeler:

Apoptoz, Hayvanlar, yenidoğan, Sıçan, Wistar, Metamfetamin, Modeller, hayvan, Modeller, teorik, Sitotoksisite, immünolojik

Cell death due to methamphetamine in primary oligodendrocyte culture

Objective: The neurotoxic effect of methamphetamine which is a psyhcostimulant drug has been established by in vitro and in vivo studies. In this study, the cytotoxic effect of mediamphetamine on oligodendrocytes has been investigated. Material and Method: The cytotoxic effect of metamphetamine was determined by lactate dehydrogenase (LDH) test in neonatal Wistar rat oligodendrocyte cultures. The apoptosis inducing effect of methamphetamine was evaluated by apostain immunfluorescence staining method. For this purpose cultures have been exposed to the various concentrations of methamphetamine (10-1000 $\mu$M with various incubation time (24-96 hours). Results LDH measures show that mediamphetamine leads dose and time-dependent oligodendrocyte death. Apostain method points that methamphetamine results in apoptotic oligodendrocyte death especially at low (10 $\mu$M and high (1000 $\mu$M doses. Conclusions: These results suggest that neurotoxic effects that are observed in chronic mediamphetamine users may be partly due to the toxic effect of methamphetamine on oligodendrocyte.

Keywords:

Apoptosis, Animals, Newborn, Rats, Wistar, Methamphetamine, Models, Animal, Models, Theoretical, Cytotoxicity, Immunologic,

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