Füsun ERTEN, Hasan GENÇOĞLU, KAZİM ŞAHİN

Deneysel Otizm Spektrum Bozukluğu Modeli Oluşturulan Sıçanlarda Likopenin Beyin IL–6, IL–10, FGF–2 ve NGF Düzeyleri Üzerine Etkisi

Otizm spektrum bozukluğu (OSB), batılı ve gelişmiş toplumlarda artan bir sorundur. Doğası gereği büyük ölçüde genetik olmasına rağmen, birçok çevresel faktör hassas popülasyonlarda OSB'yi tetiklemede rol oynayabilmektedir. Propiyonik asit (PPA) uygulaması, anormal nöral hücre organizasyonunu ve ardından otizm benzeri nörodavranışları içeren kritik değişiklikleri indükleyebilmektedir. Likopen ve metabolitleri beyinde kontrol edilebildiğinden, likopenin merkezi sinir sisteminde nöroprotektif etkileri olabileceği ve başlıca beyin biyo-belirteçleri üzerinde modülasyona neden olabileceği düşünülmektedir. Bu çalışmada, 35 adet üç haftalık yaşta Sprague Dawley ırkı erkek sıçan 5 gruba ayrıldı: i) Kontrol. ii) PPA; (500 mg/kg/ip). iii) PPA+LI (PPA’ya ek olarak, 5 mg/kg/gün intragastrik likopen verilen grup), iv) PPA+LII; (PPA’ya ek olarak, 10 mg/kg/gün intragastrik likopen verilen grup), v) PPA+LIII, (PPA’ya ek olarak, 20 mg/kg/gün intragastrik likopen verilen grup). Çalışma sonunda hayvanlar dekapite edildi ve beyin dokuları alınarak homojenize edildi ve SDS-PAGE ve western blot teknikleriyle beyinde enflamatuar sitokinler interlökin 6 ve 10’un (IL6/IL10) ile temel fibroblast büyüme faktörü (FGF-2) ve sinir büyüme faktörü (NGF) düzeylerinin değişimi tespit edildi. Elde edilen sonuçlara göre 35 günlük uygulama sonunda likopenin, PPA ile OSB modeli oluşturulan sıçanlarda, PPA’ya bağlı olarak artan IL–6 ve IL-10 düzeylerini özellikle PPA+LIII ve PPA+LII grubunda düşürdüğü tespit edildi. Bununla birlikte, FGF–2 ve NGF düzeyleri de her üç likopen grubunda da belirgin olarak PPA verilen gruba göre artış gösterdi (P

Anahtar Kelimeler:

likopen, otizm, propiyonik asit, sıçan modeli

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