Akın KOÇHAN, Aynur ŞİMŞEK, Duygu Neval SAYIN İPEK, Hasan İÇEN

Bir Buzağıda Giardia duodenalis Kaynaklı Şiddetli Kanlı İshal Olgusu

Giardia duodenalis (G. lamblia, G. intestinalis) ruminantlarda, insanlarda ve yaban hayvanlarında yaygın olarak bulunan bir protozoondur.G. duodenalis buzağılarda enteritis, kilo kaybı ve gelişme geriliğine neden olan ishalin önemli bir etiyolojik ajandır. Bu çalışmanın amacıkanlı ishale neden olan G. duodenalis’in moleküler teşhisini, klinik ve laboratuvar bulguları bildirmektir. Olgu sunumunun materyalini DicleÜniversitesi Veteriner Fakültesi Kliniğine, kanlı ishal şikâyeti ile getirilen üç aylık erkek bir buzağı oluşturdu. Buzağının yapılan klinikmuayenesinde kanlı bir ishalle birlikte, vücut sıcaklığının 39,8 0C, kalp frekansı 92 vurum/dk ve solunum sayısının 53 solunum/dk olduğutespit edildi. Tanı amacıyla rektumdan dışkı örnekleri alındı. İshale neden olan (Rotavirus, Coronavirus, Cryptosporidium, E.coli F5(K99) veCl. perfringens type) ajanların teşhisinde kullanılan hızlı tanı kiti ile bu ajanların varlığı yönünden incelendi. Bu ajanların hiçbiri tespitedilemedi, mikroskobik incelemede Giardia trofozoitleri görülürken koksidiosis etkeni olan Eimeria spp. ookistlerine rastlanılmadı. Kesintanı için parazitoloji laboratuvarına gönderilen örneğin moleküler (Nested PZR) analizinde G. duodenalis (Assemblage D) tespit edildi.Sonuç olarak; G. duodenalis assemblage D’nin de buzağılarda şiddetli kanlı ishale sebep olabileceği ve buzağılar için potansiyel bir problemolduğu kanaatine varıldı.

Severe Bloody Diarrhea in a Calf Infected with Giardia duodenalis

The parasitic protozoan Giardia duodenalis (syn. G. lamblia, G. intestinalis) is common in domestic animals, wild-life, ruminants and humans. G. duodenalis is an important etiological agent of calf diarrhea. This protozoon parasite can cause enteritis, weight loss and growth retardation in calves. The aim of this study was to reporting clinical-laboratory findings and molecular diagnosis of G. duodenalis in a severe bloody diarrheic calf. The material of this case was consisted of a three months-old male calf, which brought with complaint of severe bloody diarrhea to the Department of Internal Medicine, Faculty of Veterinary Medicine, Dicle University. The calf was suffered from severe bloody diarrhea and rectal temperature 39,8 0C, heart rate 92 beats/min. and respiratory rate 53 breaths/min. were recorded in clinical examination. For the diagnosis, feces samples were collected from the rectum of the calf. A rapid diagnostic kit for diarrhea agent was used for diagnosis of rotavirus, coronavirus, cryptosporidium, E. coli F5(K99), Cl. perfringens type. None of them were positive. Giardia cysts were detected in the macroscopical examination of feces while coccidiosis was not found. G. duodenalis (Assemblage D) was detected in the molecular (Nested PCR) analysis of the sample sent to the parasitology laboratory for definitive diagnosis. The results of this study indicated that Giardia duodenalis assemblage D may cause severe bloody diarrhea in calves and should be considered as a potential problem for calves.

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