Konkur Atlarında Egzersiz Öncesi ve Sonrası ADAMTS-4 ve ADAMTS-5 Düzeyleri

A disintegrin-like and metalloproteinase with thrombospondin motifs (ADAMTS) proteinaz ailesi, spor atlarında lokomotor sistem sağlığının korunması gibi birçok fizyolojik ve fizyopatolojik olayda önemli rol oynamaktadır. Bu çalışmada, yarış atlarında egzersiz öncesi ve sonrasındaki ADAMTS-4 ve ADAMTS-5 düzeylerinin belirlenmesi amaçlanmıştır. Bu amaçla, sağlıklı, 6-15 yaşları arasında, 650-750 kg ağırlığında, farklı cinsiyetlerden, Oldenburg ve Selle Français ırkı yarış atları (n=10) kullanıldı. Atların fiziksel muayeneleri ardından, 50 dakikalık standart egzersiz programı uygulandı. Egzersiz öncesi ve sonrasında ADAMTS-4 ve ADAMTS-5 mRNA ekspresyonlarını ve ELİSA düzeyleri ölçülmek üzere antikoagulansız tüplere kan örnekleri alındı. ADAMTS-4 mRNA ekspresyonu %25 oranında azalmasına rağmen istatistiki olarak anlamlı bulunmadı ve ELİSA düzeyinde de değişim gözlenmedi. ADAMTS-5 mRNA ekspresyonu 3.88 kat arttı (p

Anahtar Kelimeler:

ADAMTS, Agrekanaz, At Egzersiz, Metalloproteinaz

Pre- and post-exercise ADAMTS-4 and ADAMTS-5 Levels in Concur Horses

A disintegrin-like and metalloproteinase with thrombospondin motifs (ADAMTS) proteinase family play an important role in many physiological and physiopathological processes such as the maintenance of locomotor system health in sport horses. In this study, we aimed to determine the changes of ADAMTS-4 and ADAMTS-5 levels in concour horses before and after exercise. The Oldenburg and Selle Français horse-breed types which are healthy, 6-15 years old, around 650-750 kg, and distinct genders were used (n=10). Following the physical examinations, the horses were subjected to 50 minutes of regular exercise program. Blood samples were collected into anticoagulant-free tubes in order to determine ADAMTS-4 and ADAMTS-5 mRNA expression and ELISA levels before and after exercise. There were no differences were observed statistically on ADAMTS-4, neither mRNA expression in spite of 25% downregulated, nor at the ELISA levels. On the other hand, ADAMTS-5 mRNA expression was upregulated 3.88 fold (p

Keywords:

ADAMTS, Aggrecanase, Equine Exercise, Metalloproteinase,

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