Emin KARAKURT, Mushap KURU, Serpil DAĞ, Enver BEYTUT, Hasan ORAL, Hilmi NUHOĞLU, Ayfer YILDIZ

Farklı Tipteki Köpek Meme Karsinomlarında PCNA ve MMP-9 Ekspresyonlarının İmmunohistokimyasal Olarak Değerlendirilmesi

Bu çalışmanın amacı, köpeklerde görülen çeşitli meme adenokarsinomu alt tiplerinde hücre proliferasyonu ile invazyon-metastaz kapasitesi arasında anlamlı bir ilişki olup olmadığını PCNA ve MMP-9 gibi immunohistokimyasal belirteçler aracılığıyla ortaya koymaktır. Bu çalışmanın materyali, 2012-2020 yılları arasında anabilim dalımıza getirilen yaş ortalaması 8,4 yıl olan 6 Kangal ve 4 Setter köpeğinden alınan meme kanseri doku örnekleri oluşturdu. Köpeklerden alınan doku örnekleri,% 10 tamponlu formaldehit solüsyonunda tespit edildi. Rutin doku prosedürlerinden sonra Hematoksilen ve Eosin boyama için hazırlanan parafin bloklardan 5 μm kalınlığında kesitler alındı. Kesitler, kanser paternlerini belirlemek için ışık mikroskobu altında incelendi. İmmunohistokimyasal yöntem olarak Avidin-Biotin Peroxidase yöntemi kullanıldı. 10 meme karsinomu vakasından 4'ü intraduktal papiller karsinom, 4'ü miks karsinom, 1'i solid karsinom ve 1'i tübüler karsinom olarak sınıflandırıldı. Solid alt tipte en yüksek ortalama PCNA ve MMP-9 pozitif hücre sayısını bulduk. Sonuç olarak sadece solid alt tipte hücre proliferasyonu ile invazyon ve metastaz kapasitesi arasında pozitif bir ilişki olabileceğini düşündük.

Anahtar Kelimeler:

karsinom, köpek, meme, pcna, mmp-9

Immunohistochemically Evaluation of PCNA and MMP-9 Expressions in Different Types of Canine Mammary Carcinomas

The aim of this study was to evaluate reveal whether there is a significant relationship between cell proliferation and invasion-metastasis capacity in various mammary adenocarcinoma subtypes observed in dogs, by means of immunohistochemical markers such as PCNA and MMP-9. The material of this study was composed of tissue samples of mammary carcinomas taken from 6 Kangal and 4 Setter dogs with an average age of 8.4 years brought to our department between 2012-2020 years. Tissue samples from dogs were fixed in 10 % buffered formaldehyde solution. After routine tissue procedures, 5 μm thick sections were taken from paraffin blocks prepared for Hematoxylin and Eosin staining. Sections were examined under light microscope to determine the cancer patterns. Avidin-Biotin Peroxidase method was used as immunohistochemical method. 4 of 10 mammary carcinoma cases were classified as intraductal papillary carcinoma, 4 as carcinoma mixed, 1 as solid carcinoma and 1 as tubular carcinoma. We found the highest mean number of PCNA and MMP-9 positive cells in solid subtype. In conclusion, we thought that a positive relationship between cell proliferation and invasion and metastasis capacity under the solid subtype.

Keywords:

carcinoma, dog, mammary, pcna, mmp-9,

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