Rendra Leonas, Zairin Noor, Hermawan Nagar , RASYİD, Tita Husnitawati , MADJİD, Fachry Ambia TANJUNG

Sıçanlarda kurşun nanoparçacıkların inhalasyonunun mezoyapı ve nükleer faktör-kappaB ligand sisteminin aktivatör reseptörü olan osteoprotegerine etkisi

Amaç: Bu çalışma, subkronik ve kronik inhalasyona maruz bırakılan sıçanlarda Pb nanopartikül maruziyetinin nükleer faktör-kappaB ligand (OPG / RANKL) sisteminin mezoyapı ve osteoprotegerin / reseptör aktivatörünü etkileyip etkilemediğini araştırmayı amaçlamaktadır. Gereç ve Yöntem: Kırk sekiz sıçan tesadüfen sekiz gruba ayrıldı. Bir grup, açıklanmamış bir gruptur. Üç grup Pb nanopartiküllere maruz bırakılmışken 6.25; 12.5; veya 28 gün boyunca günde bir saat 25 mg / m3 uygulandı. Diğer üç grup, nanopartikül Pb'ye maruz bırakıldı; dozlar 6.25 12.5; ve 6 ay süreyle günde bir saat 25 mg / m3 tü. Bulgular: Subkronik ve kronik Pb nanopartikülleri trabeküler mezoyapıyı değiştirdi. Subkronik maruziyetin birinci ve ikinci dozu kontrol gruplarına kıyasla OPG düzeylerini önemli ölçüde arttırdığını bulduk (P <0.05). Kronik maruziyetinde, OPG düzeylerinin birinci ve ikinci dozu kontrol grubuna göre anlamlı olarak arttığını bulduk (P <0.05). Bu çalışmada, subkronik maruziyetinde ikinci ve üçüncü dozları, birinci doza veya kontrol grubuyla kıyaslandığında önemli bir (P <0.05) azalma gösterdi. Kronik maruziyette, gruplar arasında RANKL düzeylerinde anlamlı bir farklılık bulunmadı (P> 0.05). Subkronik maruziyette, OPG / RANKL oranı üçüncü dozla kontroller kıyaslandığında anlamlı olarak arttı (P <0.05) ve kronik maruziyette anlamlı farklılıklar görülmedi.Sonuç: Osteoblastın RANKL inhibisyonu ve OPG stimülasyonunun enazından bir bölümünün aracılığıyla Pb nanopartikül trabeküler kemik neoformasyonuna indüklendi.

Anahtar Kelimeler:

İnhalasyon, toksikoloji, kemiğin yeniden oluşumu

Effect of lead nanoparticles inhalation on mesostructure and the osteoprotegerin/receptor activator of nuclear factor-kappaB ligand system in rats

Purpose: This study aims to investigate whether Pb nanoparticle exposure affects the mesostructure, and osteoprotegerin/receptor activator of nuclear factor-kappaB ligand (OPG/RANKL) system in rats exposed to subchronic and chronic inhalation. Material and Methods: Forty eight rats were randomly divided into eight groups. One group is a non-exposed group. While three groups were exposed to nanoparticles Pb at the following doses 6.25; 12.5; or 25 mg/m3 an hour daily for 28 days. Another three groups were exposed to nanoparticles Pb at following doses 6.25; 12.5; and 25 mg/m3 one hour daily for 6 months. Results: Subchronic and chronic Pb nanoparticles changed trabecular mesostructure. We found that subchronic exposure significantly increased the levels of OPG at the first and second dose exposure compared to the control groups (P < 0.05). In the chronic exposure, we found that the levels of OPG significantly increased at the first and second dose exposure compared to the control group (P < 0.05). In this study significant reduction in subchronic exposure to second and third doses compared to the first dose or exposure control (P < 0.05) were shown. In chronic exposure, no significant differences in RANKL levels were found between groups (P > 0.05). In subchronic exposure, the ratio of OPG/RANKL significantly increased between the third dose exposure compared to controls (P < 0.05), with not significant differences in chronic exposure. Conclusion: Pb nanoparticle induced trabecular bone neoformation at least a part via OPG stimulation and inhibition of RANKL by osteoblast.

Keywords:

Inhalation, toxicology, bone remodeling,

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