Melike ERSÖZ, Ayşe KARATUĞ KAÇAR, İşıl SEZEKLER, Zeynep Mine COŞKUN

MCF-7 hücrelerinde borik asit uygulanan yeşil çayın ısı şok proteinlerinin ekspresyonlarına etkileri

Amaç: Çalışmanın amacı, farklı konsantrasyonlarda borik asit uygulanan yeşil çayın MCF-7 hücrelerinde ısı şoku proteini (HSP) 70 ve HSP 90 üzerine etkilerini incelemektir.Gereç ve Yöntem: Camelia sinensis (yeşil çay)’in yetiştirildiği toprağa 0 mg/m2 (grup 1), 100 mg/m2 (grup 2), 300 mg/m2 (grup 3) ve 500 mg/m2 (grup 4) borik asit sodyum tetraborat tamponu içerisinde uygulandı. Toplanan yeşil çay yaprakları kurutuldu ve 40 dakika boyunca 80° C'de suda demlendi. Sitotoksisite MTT yöntemi ile analiz edildi. Hazırlanan yeşil çay ekstraktları (250 μg/ml) MCF-7 hücrelerine uygulandı. HSP 70 ve HSP 90'ın mRNA ekspresyonları qRT-PZR ile HSP 70 ve HSP 90 protein seviyeleri Western blotting yöntemi ile belirlendi.Bulgular: 100 ve 500 mg/m2 borik asit uygulanan yeşil çay ekstraktları MCF-7 hücrelerinde HSP 90 mRNA ekspresyonunu borik asit içermeyen yeşil çay ekstraktına göre arttırdı. Borik asit uygulanmış yeşil çay ekstraktı ve borik asit içermeyen yeşil çay ekstraktının MCF-7 hücrelerindeki HSP 70 ve HSP 90 protein seviyeleri arasında herhangi bir değişiklik saptanmadı.Sonuç: Bulgularımıza göre, MCF-7 hücrelerinde HSP 70 ve HSP 90'ın protein ekspresyonları borik asit uygulanmış ve uygulanmamış yeşil çay ekstraktları ile değişmedi.

Anahtar Kelimeler:

MCF-7 hücreleri, ısı şok protein, yeşil çay, borik asit, sitotoksisite

Effects of boric-acid-applied green tea on the expressions of heat shock proteins in MCF-7 cells

Purpose: The study aims to examine the effects of boric acid at different concentrations applied to green tea on heat shock protein (HSP) 70 and HSP 90 in MCF-7 cells. Materials and Methods: Boric acid in sodium tetraborate buffer of 0 mg/m2 (group 1), 100 mg/m2 (group 2), 300 mg/m2 (group 3), and 500 mg/m2 (group 4) were applied to soil where Camelia sinensis (green tea) grown. The collected green tea leaves were dried and brewed in water at 80°C for 40 minutes. Cytotoxicity was analyzed by MTT method. The prepared green tea extracts (250 μg/ml) were applied to MCF-7 cells. The mRNA expressions of HSP 70 and HSP 90 were determined by qRT-PCR. The HSP 70 and HSP 90 protein levels were measured by Western blotting method. Results: 100 and 500 mg/m2 boric-acid-applied green tea extracts increased HSP 90 mRNA expressions in MCF-7 cells compared to green tea extract without boric acid. HSP 70 and HSP 90 protein levels did not show any change between MCF-7 cells with boric-acid-applied green tea extract and green tea extract without boric acid. Conclusion: According to our results, the protein expressions of HSP 70 and HSP 90 in MCF-7 cells changed by neither boric-acid-applied green tea extracts nor green tea extracts without any boric-acid-applied.

Keywords:

MCF-7 cells, heat shock protein, green tea, boric acid,

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