Sıçanlarda Psikolojik Strese Bağlı Oksidan Doku Hasarında

Amaç: Hipotalamus-hipofiz-gonad aksının etkisiyle, stres yanıtının cinsiyetlerarasında farklılıklar gösterdiği bilinmektedir. Bu çalışmada postmenopozaldönemde over hormonlarının yokluğunun, psikolojik stres yanıtıve oksidatif hasar üzerindeki rolünün araştırılması amaçlandı.Yöntemler: Dişi Sprague-Dawley sıçanlar (250-300 g, n=56) kontrol, taklitcerrahi ve overektomi (OVX) olarak 3 gruba ayrıldı. Anestezi sonrası taklitcerrahi ve OVX işlemi uygulandı. Cerrahi işlemden 60 gün sonra psikolojikstres oluşturmak üzere sıçanlar elektrik şokunun uygulandığı özel bir bölmeyeyerleştirildiler. Ardından 3 gün daha aynı bölmede, aynı süre ile şokuygulanmadan tutuldular. Stres uygulamalarının 10'ar dakika öncesindesıçanlara intraperitoneal yolla glukokortikoid reseptör antagonisti RU-486(10 mg/kg) veya oksitosin reseptör antagonisti atosiban (1 mg/kg) veyaserum fizyolojik verildi. Dördüncü günde delikli levha testi uygulanmasınıtakiben sıçanlar dekapite edilerek, doku ve kan örnekleri alındı.Bulgular: Psikolojik stres, RU-486 verilen gruplarda kortizol düzeylerini anlamlıolarak arttırırken atosiban verilen gruplarda azalttı. Stres uygulamasıile serum IL-1? düzeyleri artarken, serum TNF-? düzeylerinde değişiklikgözlenmedi. Stres, özellikle overektomi uygulanmış sıçanlarda mide, kolonve beyin dokularında oksidatif hasarı arttırırken (p

Role of Ovarian Hormones in Psychological Stress-induced Oxidative Organ Damage in Rats

Objective: Stress response varies with respect to gender via the hypothalamic-pituitary-gonadal axis. We aimed to investigate the effect of ovarian hormone deficiency on psychological stress response and oxidative damage. Methods: Female Sprague Dawley rats (250-300 g, n=56) were divided as control, sham, and ovariectomy (OVX) groups. Sham operation or surgical OVX were conducted under anesthesia. After 60 days, the rats were placed in a special chamber to induce psychological stress by electric shock and were kept in the same chamber for 30 min on the following 3 days. Glucocorticoid receptor antagonist RU-486 (10 mg/kg), oxytocin receptor antagonist atosiban (1 mg/kg), or saline was intraperitoneally administered 10 min before stress exposure. After the hole-board anxiety test, the rats were decapitated on the 4th day; tissue and blood samples were obtained. Results: Psychological stress increased cortisol levels in the RU-486-administered group, while cortisol levels were decreased in the atosiban-administered group. Serum interleukin (IL)-1? levels, but not TNF-? levels, were increased by inducing stress. Stress increased oxidative damage in the stomach, colon, and brain of ovariectomized rats (p

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