Centaurea fenzlii Reichardt Özütünün Antioksidan Özellikleri ve Enzim İnhibisyon Etkisinin Belirlenmesi

Bu çalışmada Centaurea fenzlii Reichardt bitkisinin antioksidan özellikleri ve çeşitli enzimler üzerine inhibe edicietkisi araştırıldı. Bu amaçla çiçeklenme döneminde toplanan Centaurea fenzlii Reichardt’ın toprak üstü kısımlarıfarklı polariteye sahip çözücülerde maserasyon yöntemi kullanılarak çözüldü ve ekstreleri elde edildi. Çalışmalarametanol ekstresi ile devam edildi. Metanol ekstresinin toplam fenolik ve flavonoid içerikleri sırasıyla, 16,72 mgGAE/g ka ve 173,16 mg KAE/g ka olarak belirlendi. Antioksidan kapasiteleri demir indirgeyici gücü (FRAP) için0,256 mmol TE/g ka, bakır indirgeyici gücü (CUPRAC) için 0,878 mmol TE/g ka, ABTS için 0,354 mmol TE/gka ve DPPH için 0,661 mmol TE/g ka olarak saptandı. Ayrıca, ekstrelerinin kolinesteraz, α-amilaz, α-glukozidazve tirozinaz enzimlerine karşı inhibe edici etkileri de belirlendi. Enzim inhibisyon etkisi sırasıyla, α-Glukozidaziçin 0,331 mmol AKE/g ka, α-Amilaz için 0,354 mmol AKE/g ka, AChE için 0,367 mmol GAE/g ka, BChE için0,878 mmol GAE/g ka ve Tirozinaz için mmol 0,256 KE/g ka olarak bulundu.

The Determination of Antioxidant Properties and Enzyme Inhibition Effect of Centaurea fenzlii Reichardt Extract

In this study, antioxidant properties of Centaurea fenzlii Reichardt plant and its inhibitory effect on various enzymes were investigated. For this purpose, aerial parts of Centaurea fenzlii Reichardt which collected during flowering time were solved in solvents having different polarity by maceration method and extracts were obtained. The study was continued with methanol extract. Total phenolic and flavonoid contents of the methanol extract were determined as 16.72 mg GAE/g ka and 173.16 mg KAE/g ka respectively. Antioxidant capacity was determined as iron reducing power (FRAP) to 0.256 mmol TE/g ka, cupper reducing power (CUPRAC) to 0.878 mmol TE/g ka, ABTS to 0.354 mmol TE/g ka and DPPH to 0.661 mmol TE/g. Additionally, enzyme inhibiting effect of extracts were also determined against to cholinesterase, α-amylase, tyrosinase and α-glucosidase inhibition. Enzyme inhibitory effects were found for α-glucosidase 0.331 mmol AKE/g ka, α-amylase 0.354 mmol AKE/g ka, AChE to 0.367 mmol GAE/g ka, BChE to 0.878 mmol GAE/g ka and tyrosinase for mmol 0.256 KE/g respectively.

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