Necmi ÖZDEMİR, Fatih Mehmet KANDEMİR, Murat YÜKSEL, Fulya BENZER

Sağlıklı ve piyometralı ineklerde eritrosit arginaz aktivitesi ve plazma nitrik oksit düzeyi

Bu çalışma sağlıklı ve piyometralı ineklerde eritrosit arginaz aktivitesi ile plazma nitrik oksit düzeylerini saptamak amacı ile yapıldı. Araştırma materyalini Fırat Üniversitesi Veteriner Fakültesi Hayvan Hastanesi Doğum ve Jinekoloji Kliniğine getirilen 10 sağlıklı ve 10 klinik olarak piyometra teşhisi konulan, değişik ırklardan, 4‐8 yaşlarında toplam 20 inek oluşturdu. İneklerin V. Jugularisten heparinli tüplere alınan kan örnekleri santrifüj edildikten sonra plazmasında nitrik oksit düzeyi, geriye kalan kısmın 3 defa serum fizyolojik ile yıkanmasından sonra elde edilen eritrositlerde de arginaz aktivitesine bakıldı. Arginaz aktivitesi Tiyosemikarbazid‐Diasetil Monoksim Üre (TDMU) metodu ile spektrofotometrik olarak ölçüldü. Hemoglobin miktarını tespit etmede Drabkin metodu, nitrik oksit düzeyi ölçmede ise enzimatik Griess yöntemi kullanıldı. Sonuç olarak sağlıklı ineklerin eritrosit arginaz aktivitelerinin piyometralı ineklere göre belirgin derecede fazla olduğu (p

Erythrocyte arginase activity and plasma nitric oxide level in healthy cows and cows with pyometra

This study was conducted to establish the erythrocyte arginase activity and plasma nitric oxide levels in healthy cows and cows with pyometra.The study material consisted of a total of 20 cows, aged between 4 and 8 years old and in various breeds, admitted to the Obstetrics and Gynaecology Clinic, Animal Hospital, Faculty of Veterinary Medicine, Fırat University. Half of the animals were diagnosed as clinically healthy, while the others were diagnosed with pyometra. Following the centrifugation of blood samples collected from V. Jugularis into heparin‐filled tubes, the plasma nitric oxide levels were measured. Arginase activity was measured in erythrocytes after washing up of the remaining portion 3 times with serum physiologic. The activity was measured spectrophotometrically with the Thiosemicarbazide‐Diacetyl Monoxime Urea (TDMU) method. Drabkin method was used to measure the haemoglobin amount, while enzymatic Griess method was used to measure the nitric oxide levels. As a result, it was found that; i) the erythrocyte arginase activities of healthy cows were markedly higher (P

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Alghamdi AS., Foster DN., Carlson CS., Troedsson MHT., 2005. Nitric oxide levels and nitric oxide synthase expression in uterine samples from mares susceptible and resistant to persistent breeding‐induced endometritis. AJRI ., 53,230–237.
Alghamdi AS., Troedsson MHT., 2002. Concentration of nitric oxide in uterine secretion from mares susceptible and resistant to chronic post‐breeding endometritis. Theriogenology, 58, 445–448.
Ash DE., 2004. Structure and Function of Arginase. ASN., 22, 2760‐2764.
Azawi OI., 2008. Postpartum uterine infection in cattle. Anim. Reprod. Sci., 105, 187–208.
Bartlett PC., Kirk JH., Wilke MA., Kaneene JB., Mather EC., 1986. Metritis complex in Michigan Holstein‐Friesian cattle: incidence, descriptive epidemiology and estimated economic impact. Prev. Vet. Med., 4, 235‐248.
Chang K., Zhang L., 2008. Steroid hormones and uterine vascular adaptation to pregnancy. Reprod. Sci., 15, 336–348.
Cook HT., Jansen A., Lewis S., Largen P., O’Donnell M., Reaveley D., Cattel V., 1994. Arginine metabolism in experimental glomerulonephritis: Interaction between nitric oxide synthase and arginase. Am. J.Physiol. Renal. Physiol., 267, 646‐653.
Cuervo H., Pineda MA., Aoki MP., Gea S., Fresno M., Girones N., 2008. Inducible nitric oxide synthase and arginase expression in heart tissue during acute trypanosoma cruzi infection in mice: arginase I is expressed in infiltrating CD68+ macrophages. J. Infect. Dis., 197, 1772‐1782.
Davies MG., Fulton GJ., Hagen PO., 1995. Clinical biology of nitric oxide. Br. J. Surg., 82, 1598‐610.
Ekerhovd E., Brannstrom M., Weijdegard B., Norstrom A., 2000. Nitric oxide synthases in the human cervix at term pregnancy and effects of nitric oxide on cervical smooth muscle contractility. Am. J. Obstet. Gynecol., 183, 610–616.
Erbaş H., Erten O., Dağlar A., İrfanoğlu ME., 2006. Meme kist sıvısı arginaz aktivitesi, ornitin ve üre düzeyleri. Turk J. Bioch., 31, 129‐134.
Fairbanks VF., Klee GG., 1986. Biochemical aspects of hematology. In: Tietz NW (Ed.): Textbook of clinical chemistry. WB Saunders Company, Philadelphia.
Frean SP., Bryant CE., Froling IL., Elliott J., Lees P., 1997. Nitric oxide production by equine articular cells in vitro. Equıne Vet. J., 29, 98–102.
Geyer JW., Dabich D., 1971. Rapid method for determination of arginase activity in tissue homogenates. Anal. Biochem., 39, 412‐417.
Gökmen SS., Yörük Y., Çakır E., Yorulmaz F., Gülen Ş., 1999. Arginase and ornithine, as markers in human non‐small cell lung carcinoma. Cancer Biochem. Biophys., 17, 125‐131.
Hallemeesch MM., Lamers WH., Deutz NE., 2002. Reduced arginine availability and nitric oxide production. Clin. Nutr., 21, 273‐279.
Howe LM., Boothe HW., 2001. Nitric oxide a review for veterinary surgeons. Vet. Surg., 30, 44‐57.
Hrabak A., Bajor T., Temesi A., Meszaros G., 1996. The inhibitory effect of nitrite, a stable product of nitric oxide (NO) formation, on arginase. FEBS Letters, 390, 203‐206.
Kandemir FM., 2009. Koyun dalak doku arginazının bazı kinetik özellikleri. Doktora tezi, Fırat Üniv. Sağ. Bil. Ens., Elazığ.
Karadaş E., Timurkan N., 2001. Koyunlarda dişi genital sistemde patomorfolojik araştırmalar II. Uterus, Serviks ve Vagina. Turk. J. Vet. Anim. Sci., 25, 27‐37.
Kepka‐Lenhart D., Ash DE., Morris SM., 2008. Determination of mammalian arginase activity. Method. Enzymol., 440, 221‐30.
Kuyumcu A., Polat Düzgün A., Özmen MM., Besler HT., 2004. Travma ve enfeksiyonda nitrik oksidin rolü. Ulus. Travma. Derg., 10, 149‐159.
Li DJ., Liu YF., Li YF., Lv Y., Pei XY., Guo DZ., 2010. Significance of nitric oxide concentration in plasma and uterine secretes with puerperal endometritis in dairy cows. Vet. Res. Commun., 34, 315–321.
Lowder MQ., 1993. Diagnosing and treating bovine postpartum endometritis. Vet. Med., 88, 474‐479.
Lyall F., Young A., Greer IA., 1995. Nitric oxide concentrations are increased in the fetoplacental circulation in preeclampsia. Am. J. Obstet. Gynecol., 173, 714‐718.
Miyamoto A., Shirasuna K., Sasahara K., 2009. Local regulation of corpus luteum development and regression in the cow: Impact of angiogenic and vasoactive factors. Domest. Anim. Endocrinol., 37, 159–169.
Mohamed SA., Fahmy AS., Mohamed TM., Hamdy SM., 2005. Urea cycle of Fasciola gigantica: Purification and characterization of arginase. Comp. Biochem. Physiol. B Biochem. Mol. Biol., 142, 308‐316.
Moreno‐Vivian C., Soler G., Castillo F., 1992. Arginine catabolism in the phototophic bacterium Rhodobactercapsulatus E1F1. Eur. J. Biochem., 204, 531‐537.
Pegg AE., Mccann P., 1982. Polyamine metabolism and function. Am. J. Physiol. Cell. Physiol., 243, 212‐221.
Pegg AE., Mccann P., 1988. Polyamine metabolism and function in mammalian cells and protozoans. ISI Atlas Sci. Biochem., 1, 11‐18.
Polat B., 2008. İneklerde postpartum uterus enfeksiyonları. Atatürk Üniversitesi Vet. Bil. Derg., 3, 56–63.
Spector EB., Rice SCH., Cederbaum SD., 1983. Immunologic studies of arginase in tissues of normal human adult and arginase‐deficients patients. Pediatr. Res., 17, 941‐944.
Thurmond MC., Jameson CM., Picanso JP.,1993. Effect of intrauterine antimicrobial treatment in reducing calving to conception interval in cow with endometritis. J. Am. Vet. Med. Assoc., 203, 1576‐1578.
Wu CW., Wang SR., Chang TJ., Lin EC., Chang KL., Huang MH., Lui WY., P'eng FK., Chi CW., 1989. Content of glucocorticoid receptor and arginase in gastric cancer and normal gastric mucosal tissues. Cancer., 64, 2552‐2556.
Wu G., Morris SM., 1998. Arginine metabolism: nitric oxide and beyond. Biochem. J., 336,1‐7.