Koyun‐keçi çiçeği hastalığının polimeraz zincir reaksiyonu ile tanısında farklı klinik örneklerin kullanımı

Koyun‐keçi çiçeği hastalığının hızlı ve kesin tanısı, hastalığın kontrolünde oldukça önemlidir. Bu çalışmada, farklı klinik örneklerden Koyun‐keçi çiçeği virüsünün teşhisinde polimeraz zincir reaksiyonunun (PZR) etkinliğinin belirlenmesi amaçlanmıştır. Bu amaçla, Erzurum ve çevre illerde klinik olarak koyun‐keçi çiçeği hastalığı bildirimi yapılmış 11 mihraktaki 53 hayvandan 168 adet materyal temin edildi. PZR sonuçlarına göre; deri örneklerinin % 96,2 (51/53)’sinde, nazal ve oral sıvap örneklerinin % 76,9 (40/52)’unda, akciğer örneklerinin % 90,4 (19/21)’ünde, karaciğer örneklerinin % 42,8 (9/21)’inde ve lenf yumrusu örneklerinin % 61,9 (13/21)’unda pozitiflik tespit edildi. Sonuç olarak, koyun‐keçi çiçeği virüsünün PZR ile tanısında deri ve akciğer örneklerinin kullanımının uygun olduğu görülmüştür.

Use of different clinical samples in detection with polymerase chain reaction of sheep‐ goatpox disease

Rapid and accurate diagnosis of sheep‐goatpox disaese is very important for controling the disease. The aim of this study was to evaluate the use of polymerase chain reaction (PCR) in detection of sheep‐goatpox virus from different clinical samples. For this purpose, 168 clinical specimens of 53 animals from 11 outbreaks in Erzurum and its surroundings were collected. According to the PCR results,sheep‐goatpox virus DNA was detected from 96.2 % (51/53), 76.9 % (40/52), 90.4 % (19/21), 42.8 % (9/21) and 61.9 % (13/21) of cutaneous, nasal and oral samples, lung, liver and lymph nodules, respectively. As a result, it is concluded that the use of cutaneous and lung samples is suitable for diagnosis of sheep‐goatpox virus with PCR.

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