Koç spermasının dondurulmasında antioksidanların etkisi

Antioksidanlaretkimekanizmasınagöreikitürlüdür.Birincigrupta,otooksidasyonzincirinikırarakoksidasyonugeciktirenantioksidanlarbulunurkibunlaraprimerantioksidanlardenir.İkincigruptakilerisesekunderantioksidanlarolupoksidasyonunbaşlamasınıengelleyenantioksidanlardır.Kimiantioksidanlarisebirdenfazlaetkimekanizmasınasahipolduklarındançokfonksiyonluantioksidanlarolarakadlandırılırlar.Antioksidatifsavunmamekanizmaları;A,E,Cvitaminleri,β-karoten,indirgenmişglutatyon(GSH)gibibazıvitaminvekimyasalmaddelerileçeşitliantioksidanenzimlerdenoluşur.Evitamini,yapısındakifenolikhidroksilgrubundandolayıgüçlübirantioksidanözellikgösterir.ZincirkırıcıantioksidatifetkinliğiilemembranfosfolipitlerindebulunanPUFAyıserbestradikalhasarındankoruyanilksavunmahattınıoluşturur.Enönemliantioksidanenzimler;süperoksitanyonunuH 2O2yedönüştürensüperoksitdismutaz(SOD),organikperoksitleridetoksifiyeedenglutatyonperoksidaz(GSH-Px)veH 2O2yisuyaindirgeyenkatalazdır.Koçspermasıdondurmayakarşıoldukçahassastır.Budurumuntemelnedenispermatozoamembranınınönemlibirkısmınıdoymamışyağasitlerinin(fosfolipitler)oluşturmasıdır.Dolayısıylaspermanındondurulmasısırasındaspermatozoonmembranıhasarauğramaktadır.Budafertilizasyonaçısındanolumsuzsonuçlardoğurmaktadır.Antioksidanlarisespermasulandırıcılarınakatılmaksuretiyledondurmasürecindeoluşabilecekhasarlarıminimizeetmektedirler.

Effect of antioxidants on cryopreservation of ram semen

Antioxidants are two types in terms of mechanism of their action. In the first group, the antioxidants retard theoxidation by breaking the autoxidation chain, called primary antioxidants. Whereas, the second group are secondaryantioxidants that prevent the onset of oxidation. Because some antioxidants have multiple mechanisms of action, they arecalled as multi-functional antioxidants. Antioxidative defence mechanisms comprise some vitamins, chemical substancesand various antioxidant enzyme, such as vitamins A, E, C, β-carotene, reduced glutathione (GSH). Vitamin E shows strongantioxidant property due to the phenolic hydroxyl group in its structure. It builds the first defence line that protects fromfree radical damage the PUFA in membrane phospholipids by chain breaker antioxidative activity. The most importantantioxidant enzymes are superoxide dismutase (SOD) that converts the superoxide anion to H 2O2, glutathione peroxidase(GSH-Px) that detoxifies the organic peroxides and catalase that reduces the H 2O2 to H 2O. Ram semen is rather sensitiveagainst the cryopreservation, since the unsaturated fatty acids constitute the major part of sperm membrane. During thesemen cryopreservation processes, sperm membrane suffers from damage. This causes to deteriorated architecture interms of fertilisation. Supplementation of antioxidants minimises the damages related with cryopreservation of semen.

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