İnsan İskeleti Parçalarından ve Diş Örneklerinden Amelogenin Genini Kullanarak Cinsiyet Belirleme

DNA örneklerindeki cinsiyet tayini için alternatif yaklaşımlardan biri de Amelogenin gen bölgeleridir. X ve Y kromozomlarının üzerinde bulunan bu gen diş minesini de kodlar. Medline araştırma sitesinde makale ve deneyimler taranarak kendi deneyimlerimizin dışında farklı araştırmalarda gözden gecirilmektedir. Mevcut tekniklerle cinsiyet tayini başarısızlıkları seyreklik gösterirken, Amelogenin gen yöntemi tek başına başarılı değildir. Amelogenin PCR yötemi/sisteminde cinsiyet tayini şu anda geleneksel yöntemin yerini almamalıdır. Bu nedenle özellikle adli amaçlı konularda Amelogenin geniyle cinsiyet tayininin yanılınabilirliğini önlemek için bir çok morfolojik-moleküler yöntemle birleştirilerek yapılmalıdır.

Anahtar Kelimeler:

Amelogenin geni, kemik, diş, cinsiyet

The Use of Amelogenin Gene in Sex Determination from Human Skeletal Fragments and Teeth Specimens

Alternative approaches to sex determination of DNA samples involve investigation of regions of the amelogenin gene. This is the gene that encodes tooth enamel and is present on both the X and Y chromosomes. A review composed via Medline Internet search of literature and contributions from our experiences as well as experiences from colleagues. The rareness of failures in sex determination provides confidence in current techniques, but amelogenin gene method (singly) of sex determination is not without failures. Amelogenin PCR method/system of sex determination should not, at the moment, completely replace traditional methods of sex identification. Hence, sex identification with amelogenin gene, of subjects for forensic purposes should be conducted as much as possible through a multiple morphological-molecular combined methods to avoid fallibility of amelogenin gene.

Keywords:

Amelogenin bone, tooth, sexing, review.,

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