İlhami ÇELİK, Mustafa CİHANGİROĞLU, Pınar YÜCE, Ayhan AKBULUT, S. Sırrı KILIÇ

Hastane kökenli metisilin dirençli stafilokok suşlarında slime oluşumu ile bunun teikoplanin duyarlılığına etkisinin araştırılması

Nozokomiyal pnömoni ve sepsis etkeni olarak izole edilen 50 stafilokok suşunda E-test yöntemiyle ölçülen teikoplanin minimum inhibitör konsantrasyonu (MİK) değerleri ile Kongo red agar plak yöntemiyle slime faktörü oluşumu arasında ilişki olup olmadığı araştırılmıştır. Çalışmaya alınan 50 suşun 35’i Staphylococcus aureus, 15’i koagülaz negatif stafilokok (KNS) idi. Suşların 31’i endotrakeal aspirattan (ETA), 19’u kandan izole edilmiştir. Otuzbir S.aureus ve 12 KNS suşu slime oluşturmuş, 4 S.aureus ve 3 KNS suşu oluşturmamıştır. Teikoplanine birer S.aureus ve KNS suşu orta duyarlı bulunmuş, dirençli suşa rastlanmamıştır. S.aureus veya KNS suşları için, ETA veya kandan izole edilenler için, slime oluşturan veya oluşturmayan suşlar için teikoplaninin ortalama MİK değerleri arasında anlamlı bir fark bulunmamıştır (p>0.05). Yine de ortalama MİK değerinin slime oluşturan suşlarda (4.3$pm$3.5 µg/ml) oluşturmayanlardakinden (2.7$pm$1.2 µg/ml) yüksek olması konunun daha fazla suşla incelenmesi gerektiğini düşündürmüştür.

Anahtar Kelimeler:

İlaç direnci, Staphylococcus, Metisilin direnci, Çapraz enfeksiyon, Mikrobiyal duyarlılık testleri, Teikoplanin

Investigation of slime production of nosocomial methicillin-resistant staphylococci and its relationship to teicoplanin susceptibility

In 50 Staphylococcus strains, isolated as the causal agents of nosocomial pneumonia and sepsis, the slime production and teicoplanin MIC’s were determined by Kongo red agar plate method and E-test, respectively, and the correlation between these two parameters were analyzed. Thirty-five strains were S.aureus and 15 strains were coagulase negative staphylococci (CNS). Thirty-one of the strains were isolated from endotracheal aspirates (ETA) and 19 from blood. Thirty-one S.aureus and 12 CNS strains were found as slime producer, and 4 S.aureus and 3 CNS strains as nonproducer. One S.aureus and 1 CNS strains were found moderately susceptible to teicoplanin to which no resistant strain was encountered. No significant difference was found for the mean teicoplanin MIC’s between S.aureus and CNS strains, or between the strains from ETA or blood, or between the slime producers and nonproducers (p>0.05). But since the mean MIC washigher in producers (4.3$pm$3.5 µg/ml) than that in nonproducers (2.7$pm$1.2 µg/ml), although insignificantly, it is concluded that such a correlationshould be investigated with higher numbers of strains.

Keywords:

Drug Resistance, Staphylococcus, Methicillin Resistance, Cross Infection, Microbial Sensitivity Tests, Teicoplanin,

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