Handan ÖZDEMİR, Kadir TÜRKÖLMEZ, Murat BOZLU, Sadettin KÜPELİ

Prostatik lezyonlarda nukleolar organizer regions (NORs) değerinin yeri ve prostat adenokanserinde tümör evre, grade ve prostat spesifik antijen dansitesi (PSAD) ile karşılaştırılması

Tümör diferansiyasyonu ve proliferatif aktivitesi, tümörün biyolojik davranışının önemli göstergeleridir. Rutin histolojik değerlendirmelerle bu parametrelerin ölçümü yetersiz olmaktadır. Silver staining nucleolar organizer regions (AgNOR) tümörün diferansiasyonunun ölçümünde yardımcı bir yöntemdir. Ondokuz hiperplastik, 31 intraepiteliyal neoplastik (PIN) ve 30 malign prostatik lezyonu olan vakada AgNOR metodu çalışılmıştır. Her vaka için ortalama AgNOR değerleri hesaplanmıştır. BPH'lı vakalarla karşılaştırıldığında, PIN ve prostat kanserli (Prostat Ca) gruplarda ortalama AgNOR değeri belirgin olarak yüksek bulunmuştur (p

Nucleolar organizer regions (NORs) in prostatic lesions, comparison with tumor grade, stage and prostatic spesific antigen density (PSAD) in prostate adenocarcinoma

Tumor differentiation and proliferative activity are important predictors of biological behavior while routine histological evaluation is fairly adequate to assess differentiation, tumor proliferative activity is difficult to measure. Silver staining for nucleolar organizer regions (AgNORs) is reported to be helpful for assessing tumor differentiation. Silver-stained nucleolar organizer regions (AgNORs) were studied in 19 hyperplasic, 31 intra-epithelial neoplastic and 30 malignant prostatic lesions. The mean AgNOR count per case was calculated. Mean AgNOR count was significantly higher (p

PDF

___

1. Darnell J, Lodish H, Baltimore D. Moleculer celi biology, New York, Scientific American Books 1986; 269-369.
2. Ferguson-Smith M.A, Handmaker SD. Observations on the satellited human chromosomes, Lancet 1961; 638-640.
3. Crocker J. Nucleolar organizer regions. İn: Undervvood JCE, ed. Pathology of Nucleus. London: Springer-Verlof, 1990; 158: 227-232.
4. Crocker J, Skilbeck N. Nucleolar organizer region associ-ated proteins in cutaneous melanotic lesions: A quan-titative study, J Clin Pathol 1987; 40: 885-889.
5. Egan MJ. Raafat F, Crocker J ve ark. Nucleolar organizer re-gions in small celi tumors of childhood, J Pathol 1987; 153: 275-280.
6. Homat AJ, Giri DD, Cotton DVVIC ve ark. Nucleolar organi-zer regions in Spitz nevi and malignant melanomas, Cancer 1989; 63: 474-478.
7. Crocker J, Nar P. Nucleolar organizer regions in Lympho-mas, J Pathol 1987; 151: 111-118.
8. Smith R, Crocker J. Evaluation of the nucleolar organizer re-giorı-associated protein in breast malignancy, Histo-pathology 1988; 12: 113-125.
9. Ramsden AL, Murry P. Nucleolar organizer regions in cer-vical squamous pathology, J Clin Pathol 1989; 42: 781-782.
10. Ooms ECM, Veldhuizen RW. Argyrophilic proteins of the nucleolar organizer regions in bladder tumors, Virc-hovvs Arch (A) 1989; 414: 365-369.
11. Griffiths AP, Butler CW, Roberts P ve ark. Silver stained structures (AgNORs): Their dependence and tissue fi-xation and absence of prognostic relevance in rectal adenocarcinoma, J Pathol 1989; 159: 121-127.
12. Hansen AB, Ostergard B. Nucleolar organizer regions in hyperplastic and neoplastic prostatic tissue, Virchovvs Arch (A) 1990; 17: 9-13.
13. Deschenes J, VVeidner N. Nucleolar organizer regions (NOR) in hyperplastic and neoplastic prostate disease, Am J Clin Pathol 1990; 14/12: 1148-1155.
14. Bravvn PN, Ayala AG, Van Eschenbach A ve ark. Histologic grading study of prostate carcinoma, Cancer 1982; 49: 525-528.
15. Delahınt B, Bethvvaite PB, Nacey JN ve ark. Proliferating celi nuclear antigen (PCNA) expression as a prognostic indicator for renal celi carcinoma: Comparison with tumor grade, mitotic index and silver-staining nucle-olar organizer regions numbers, J Pathol 1993; 170: 471-477.
16. Yang AH, Wang TU, Lıu HC. Comparative study of the prognostic value of nucleolar grade and silver-binding nucleolar organizer region in renal celi carcinomas, J Pathol 1992; 166: 157-161.
17. Muller M, Lauke H, Hartmann M. The value of the AgNOR staining method in identifying carcinoma in sitıı testis, Path Res Pract 1994; 190: 429-435.
18. Gleason DF. Histologic grading of prostate cancer. A pros-pective, Hum Pathol 1992; 23: 273-279.
19. Gleason DF. Histological grading and clinical staging of prostatic carcinoma. İn: Tannenbaum M ed. Urologic Pathology, The prostate. Philadelphia: Lea and Febi-ger, 1977; 171-197.
20. Ploton D, Menager M, Jeannesson P ve ark. Improvement in staining and in the visualisation of argyrophilic prote-ins of the nucleolar organizer region at optical level, Histochem J 1986; 18: 5-14.
21. Crocker J, Boldy DAR, Egan MJ. Hovv should we count Ag-NORs? Proposals for a standardised approach, J Pathol 1989; 158: 185.
22. Leek RD, Alison MR, Samat CE. Variations in the occurren-ce of silver-staining nucleolar organizer regions (Ag-NORs) in non-proliferating and proliferating tissues, J Pathol 1991; 165(1): 43-51.
23. Sakr WA, Sarkar FH, Srepathi ve ark. Measurement of cellu-lar proliferation in human prostate by AgNOR, PCNA and SPF, Prostate 1993; 22(2): 147-154.
24. Lloyd SN, Johnson CP, Brown İL ve ark. Nucleolar organi-zer regions in benign and malignant prostatic disease, Histopathology 1991; 18(5): 449-452.
25. Pavlakis K, Alivizatos G, Mitropoulos D ve ark. Silver bin-ding nucleolar organizer regions in benign and malig-nant prostatic lesions, Urol Int 1992; 49(3): 137-140.
26. Mamaeva S, Lundgren R, Eltving P ve ark. AgNOR staining in benign hyperplasia and carcinoma of the prostate, Prostate 1991; 18(2): 155-62.
27. Hansen AB, Ostergard B. Nucleolar organizer regions in hyperplastic and neoplastic prostatic tissue, Virchovvs Arch A Pathol Anat Histopathol 1990; 417(1): 9-13.
28. Alivizatos G, Pavlaki K, Giannopoulos ve ark. Nucleolar or-ganizer regions in prostatic adenocarcinomas. Com-parison vvith flovv cytometric analysis, tumor grade, stage and serum prostate-spesific antigen levels, Eur Urol 1992; 21(2): 141-145.
29. Deschenes J, VVeidner N. Nucleolar organizer regions (NOR) in hyperplastic and neoplastic prostate disease, Am J Surg Pathol 1990; 14: 1148-1155.