Aydın KARAARSLAN, Özlem ÖZDEMİR, İlkay BOYACIOĞLU

M. pneumoniae' nin insan alveoler makrofajlarına tutunması

Hemadsorbsiyon pozitif (HA+) ve cam yüzeylere tutunabilen MP129 M. pneumoniae suşıı ile bu özellikleri göstermeyen M. pneumoniae FH suşunun insan alveoler makrofajlarına tutunmaları ELISA yöntemi ile araştırılmıştır. HA+ M. pneumoniae suşlarının hemadsorbsiyon negatif (HA-) olanlara göre makrofajlara daha fazla bağlandıkları ve opsonizasyonun tutunmayı olumlu yönde etkilediği tespit edilmiştir. Bu çalışmada, M. pneumoniae infeksiyonundan korunmada alveoler makrofajların bir savunma aracı olduğu ve aderans ve fagositoz çalışmalarında ELISA'nın daha kolay ve basit bir teknik olduğu sonu c una varılrnıştır.

Adherence of Mycoplasma pneumoniae to human alveolar macrophages

Attachment to alveoler macrophages of M. pneumoniae M129 strain which is HA positive and glass-.adhering and M. pneumoniae FH strain which does not show these features was investigated by ELISA. It was determined that the glass-adhering, and HA pozitive M. pneumoniae M 129 strain was attached more to human alveoler macrophages when compared with the non glass-adhering and HA negative M. pneumoniae FH strain and the treatment of the bacteria with normal human serum was enhanced the attachment of both strain. In this study, it was concluded that the human alveoler macrophages may elicit a protective response agaiııst M. pneumoniae infection, and ELISA is easy and simple method in adherence and phagocytosis studies.

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