Ergul MUTLU ALTUNDAG, Eda BECER, Duygu GENCALP, Seda VATANSEVER

KUERSETİN, KURKUMİN VE KOMBİNASYONLARININ MEME KANSERİ HÜCRE HATLARI ÜZERİNDEKİ ANTİPROLİFERATİF ETKİLERİNİN ARAŞTIRILMASI

Amaç: Meme kanseri, kadınlarda malignite ve mortalite oranı yüksek olan ve Doğu Akdeniz bölgesinde sıklıkla görülen bir kanser türüdür. Kuersetin ve kurkumin, kanser hücrelerinin büyümesini inhibe etme özelliği olduğu bilinen flavonoid türleridir. Bu çalışmanın amacı, antikanser özelliğe sahip olduğu bilinen kuersetin ve kurkumin flavonoidlerinin tek başına ve kombine kullanımının metastatik ve metastatik olmayan meme kanser hücre hatlarındaki antiproliferatif etkisinin belirlenmesidir.

THE INVESTIGATION OF ANTIPROLIFERATIVE EFFECTS OF QUERCETIN, CURCUMIN AND THEIR COMBINATIONS ON BREAST CANCER CELL LINES

Objective: Breast cancer is a type of cancer that has high malignancy and mortality rates in women and is frequently seen in the Eastern Mediterranean region. Flavonoids such as quercetin and curcumin are known to have an ability to inhibit the growth of cancer cells. The aim of this study was to determine the antiproliferative effect of quercetin and curcumin which are flavonoids known to have anticancer properties on human metastatic and non metastatic breast cancer cell lines of alone and in combination usage.

Keywords:

Antiproliferative effect, quercetin, curcumin, breast cancer,

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1. Akkuzu, M.Z., Küçüköner, M., Irtegun, S et al. (2019). Meme Kanserinde Brca-1 ve Brca-2’ de Sık Görülen Polimorfizm Mutasyonların Bölgemizde Varlığı. Dicle Med J, 46, 623–631 .
2. Elbachiri, M., Fatima, S., Bouchbika, Z et al. (2017). Breast cancer in men: About 40 cases and literature review. Pan Afr. Med. J, 28, 287.
3. Foley, N.M., Coll, J.M., Lowery, A.J et al. (2018). Re-Appraisal of Estrogen Receptor Negative/Progesterone Receptor Positive (ER−/PR+) Breast Cancer Phenotype: True Subtype or Technical Artefact? Pathol. Oncol. Res, 24, 881–884.
4. Anderson, W.F, Chatterjee. N., Ershler, W.B, Brawley, O.W. (2002). Estrogen receptor breast cancer phenotypes in the Surveillance, Epidemiology, and End Results database. Breast Cancer Res. Treat, 76, 27–36.
5. Nadji, M., Gomez-Fernandez, C., Ganjei-Azar, P., Morales, A.R. (2005). Immunohistochemistry of Estrogen and Progesterone Receptors Reconsidered. Am. J. Clin. Pathol, 123, 21–27.
6. Erić, I., Erić, A.P., Kristek, J., Koprivčić, I., Babić, M. (2018). Breast cancer in young women: Pathologic and immunohistochemical features. Acta Clin. Croat, 57, 497–502.
7. Kampa, M., Nifli, A.P., Notas, G., Castanas, E. (2007). Polyphenols and cancer cell growth. Rev. Physiol. Biochem. Pharmacol, 159, 79–113.
8. Birman, H. (2012). Bitkisel Flavonoid Bileşiklerinin Biyoaktiviteleri ve Muhtemel Etki Mekanizmaları. İstanbul Tıp Fakültesi Derg, 75, 46–49.
9. Slimestad, R., Fossen, T., Vågen, I.M. (2007). Onions: A source of unique dietary flavonoids. J Agr Food Chem, 55, 10067–10080.
10. Spagnuolo, C., Russo, M., Bilotto, S., Tedesco, I., Laratta, B., Russo, G.L. (2012). Dietary polyphenols in cancer prevention: the example of the flavonoid quercetin in leukemia. Ann. N. Y. Acad. Sci, 1259, 95–103.
11. Nam, J.S., Sharma, A.R., Nguyen, L.T., Chakraborty, C., Sharma, G., Lee, S. (2016). Application of bioactive quercetin in oncotherapy: From nutrition to nanomedicine. Molecules, 21(1), E108.
12. Mutlu Altundağ, E., Yılmaz, A.M., Kocturk, S., Taga, Y., Yalçın, A.S. (2017). Synergistic Induction of Apoptosis by Quercetin and Curcumin in Chronic Myeloid Leukemia (K562) Cells. Nutr Cancer, 70, 97-108.
13. Nagahama, K., Utsimi, T., Kumano, T., Maekawa, S., Oyama, N., Kawakami, J. (2016). Discovery of a new function of curcumin which enhances its anticancer therapeutic potency. Sci. Rep, 6, 30962.
14. Troselj, K., Kujundzic, R. (2014). Curcumin in Combined Cancer Therapy. Curr. Pharm. Des, 20, 6682–6696.
15. Kunnumakkara, A.B., Bordoloi, D., Padmavathi, G et al. (2017). Curcumin, the golden nutraceutical: multitargeting for multiple chronic diseases. Br. J. Pharmacol, 174, 1325–1348.
16. Comşa, Ş., Cîmpean, A.M., Raica, M. (2015). The story of MCF-7 breast cancer cell line: 40 Years of experience in research. Anticancer Res, 35, 3147–3154.
17. Sweeney, E.E., McDaniel, R.E., Maximov, P.Y., Fan, P., Craig Jordan, V. (2012). Models and mechanisms of acquired antihormone resistance in breast cancer: Significant clinical progress despite limitations. Horm. Mol. Biol. Clin. Investig, 9, 143–163.
18. Urquidi, V., Sioan, D., Kawai, K et al. (2002). Contrasting expression of thrombospondin-1 and osteopontin correlates with absence or presence of metastatic phenotype in an isogenic model of spontaneous human breast cancer metastasis. Clin. Cancer Res, 8, 61–74 .
19. Goodison, S., Viars, C., Grazzini, M. & Urquidi, V. (2003). The interrelationship between DRIM gene expression and cytogenetic and phenotypic characteristics in human breast tumor cell lines. BMC Genomics, 4, 39.
20. Srivastava, N.S., Srivastava, R.A.K. (2019). Curcumin and quercetin synergistically inhibit cancer cell proliferation in multiple cancer cells and modulate Wnt/β-catenin signaling and apoptotic pathways in A375 cells. Phytomedicine, 52, 117–128 .
21. Chou, T.C. (2010). Drug combination studies and their synergy quantification using the Chou-Talalay method. Cancer Res, 70, 440–446.
22. Li, X., Zhou, N., Wang, J et al. (2018). Quercetin suppresses breast cancer stem cells (CD44+/CD24−) by inhibiting the PI3K/Akt/mTOR-signaling pathway. Life Sci, 196, 56–62.
23. Ranganathan, S., Halagowder, D., Sivasithambaram, N.D. (2015). Quercetin Suppresses Twist to Induce Apoptosis in MCF-7 Breast Cancer Cells. PLoS One, 10(10), e0141370.
24. Liu, J.L., Pan, Y.Y., Chen, O et al. (2017). Curcumin inhibits MCF‑7 cells by modulating the NF‑κB signaling pathway. Oncol. Lett, 14, 5581–5584.